Nikitin A I, Kitaev E M, Savitskiĭ G A, Ivanova R D, Kalashnikova E P
Arkh Anat Gistol Embriol. 1987 Oct;93(10):39-43.
With the aim to use a previously elaborated method of extracorporeal fertilization of human ova for treating sterility, resulted from obstruction or absence of the uterine tubes, ova were obtained at laparoscopy after a previous hormonal stimulation of women. From 119 women 159 ova were obtained. Fertilization of the ova was performed in the medium Ham F-10 previously capacitated with the husband's spermatozoa. Fertilization rate was 66%. Embryos at the stage of 2-8 blastomeres were implanted transcervically into the uterine cavity with a special catheter. The embryo transfer was performed in 37 women with tubular infecundity. Menoschesis up to 3 weeks was noted in 5 women. In one woman on the 35th day after implantation, by means of hormonal-biological reaction and ultrasound investigation, pregnancy was diagnosed, it terminated in timely birth of a boy with body mass 3,500 gr. A conclusion was made that the main cause of failures at the embryo transfer was an inadequate preparation of the uterine mucosa (resulted from the main disease) to accept the fertilized ovum.
为了运用先前精心设计的人类卵子体外受精方法来治疗因输卵管阻塞或缺失导致的不育症，在对女性进行先前的激素刺激后，通过腹腔镜检查获取卵子。从119名女性中获取了159枚卵子。卵子在预先用丈夫精子进行获能处理的哈姆F-10培养基中进行受精。受精率为66%。将处于2-8细胞期的胚胎通过特殊导管经宫颈植入子宫腔。对37名输卵管性不孕的女性进行了胚胎移植。5名女性出现了长达3周的闭经。在1名女性移植后第35天，通过激素-生物学反应和超声检查诊断出怀孕，最终顺利产下一名体重3500克的男婴。得出的结论是，胚胎移植失败的主要原因是子宫黏膜（由主要疾病导致）准备不足，无法接受受精卵。
