Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München (GmbH), Neuherberg, Germany.
Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München (GmbH), Neuherberg, Germany; Center for Translational Cancer Research (TranslaTUM), School of Medicine, Technical University of Munich, Munich, Germany.
Methods Enzymol. 2021;657:365-383. doi: 10.1016/bs.mie.2021.06.031. Epub 2021 Jul 21.
Photochromic proteins and photoswitching optoacoustics (OA) are a promising combination, that allows OA imaging of even small numbers of cells in whole live animals and thus can facilitate a more wide-spread use of OA in life-science and preclinical research. The concept relies on exploiting the modulation achieved by the photoswitching to discriminate the agents' signal from the non-modulating background. Here we share our analysis approaches that can be readily used on data generated with commercial OA tomography imaging instrumentation allowing-depending on the used photoswitching agent and sample-routine visualizations of as little as several hundreds of transgene labeled cells per imaging volume in the live animal.
光致变色蛋白和光开关光声（OA）成像技术是一种很有前途的组合，它可以对整个活体动物中甚至少量的细胞进行 OA 成像，从而可以促进 OA 在生命科学和临床前研究中的更广泛应用。该概念依赖于利用光致变色所实现的调制来区分试剂的信号与非调制背景。在这里，我们分享我们的分析方法，这些方法可以很容易地应用于使用商业 OA 断层扫描成像仪器生成的数据，根据所使用的光致变色试剂和样本，在活体动物的每个成像体积中，对数量少至几百个转基因标记细胞进行常规可视化。
