利用可反复切换的光声蛋白进行多重整体动物成像。

Institute of Biological and Medical Imaging (IBMI), Helmholtz Zentrum München, Neuherberg, Germany.

Institute for Medical Microbiology, Immunology and Hygiene, Technische Universität München (TUM), Munich, Germany.

Sci Adv. 2020 Jun 12;6(24):eaaz6293. doi: 10.1126/sciadv.aaz6293. eCollection 2020 Jun.

We introduce two photochromic proteins for cell-specific in vivo optoacoustic (OA) imaging with signal unmixing in the temporal domain. We show highly sensitive, multiplexed visualization of T lymphocytes, bacteria, and tumors in the mouse body and brain. We developed machine learning-based software for commercial imaging systems for temporal unmixed OA imaging, enabling its routine use in life sciences.

我们介绍了两种光致变色蛋白，可用于具有时间域信号解混的细胞特异性活体光声（OA）成像。我们展示了在小鼠体内和大脑中对 T 淋巴细胞、细菌和肿瘤的高灵敏度、多重可视化。我们为商用成像系统开发了基于机器学习的时间解混光声成像软件，使其能够在生命科学中常规使用。