鉴定蜕皮激素25 - O - β - D - 吡喃葡萄糖苷为马副蛔虫中线虫蜕皮激素的一种新代谢产物。
Identification of ecdysone 25-O-beta-D-glucopyranoside as a new metabolite of ecdysone in the nematode Parascaris equorum.
作者信息
O'Hanlon G M, Howarth O W, Rees H H
机构信息
Department of Biochemistry, University of Liverpool, U.K.
出版信息
Biochem J. 1987 Nov 15;248(1):305-7. doi: 10.1042/bj2480305.
A major metabolite of [3H]ecdysone injected into adults of the nematode Parascaris equorum maintained in vitro for 48 h was secreted into the culture medium. The compound could be hydrolysed with a crude hydrolase preparation from Helix pomatia, yielding ecdysone. Sufficient quantity of this material for identification was produced by administration of ecdysone to the parasites. The resulting compound was purified by h.p.l.c. and identified as ecdysone 25-O-beta-D-glucopyranoside by n.m.r. spectroscopy and by fast atom bombardment mass spectrometry of the conjugate and of the sugar released by enzymic hydrolysis. The significance of formation of the glucoside is uncertain.
将[3H]蜕皮激素注射到体外培养48小时的马副蛔虫成虫体内后，其一种主要代谢产物分泌到了培养基中。该化合物可用来自苹果螺的粗水解酶制剂进行水解，生成蜕皮激素。通过给寄生虫施用蜕皮激素，产生了足以用于鉴定的该物质的量。所得化合物通过高效液相色谱法进行纯化，并通过核磁共振光谱以及对缀合物和酶促水解释放的糖进行快原子轰击质谱分析，鉴定为蜕皮激素25 - O - β - D - 吡喃葡萄糖苷。葡萄糖苷形成的意义尚不确定。
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