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1
Identification of the 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone from newly laid eggs of the desert locust, Schistocerca gregaria.从沙漠蝗虫（Schistocerca gregaria）新产的卵中鉴定蜕皮激素、2-脱氧蜕皮激素、20-羟基蜕皮激素和2-脱氧-20-羟基蜕皮激素的22-磷酸酯。
Biochem J. 1983 Aug 1;213(2):533-41. doi: 10.1042/bj2130533.
2
Isolation and identification of ecdysteroid phosphates and acetylecdysteroid phosphates from developing eggs of the locust, Schistocerca gregaria.从沙漠蝗（Schistocerca gregaria）发育中的卵中分离并鉴定蜕皮甾醇磷酸酯和乙酰蜕皮甾醇磷酸酯。
Biochem J. 1984 Jul 15;221(2):459-64. doi: 10.1042/bj2210459.
3
Isolation and identification of 3-acetylecdysone 2-phosphate, a metabolite of ecdysone, from developing eggs of Schistocerca gregaria.从沙漠蝗发育中的卵中分离并鉴定蜕皮激素的一种代谢产物——3-乙酰蜕皮激素2-磷酸酯。
Biochem J. 1984 Jan 1;217(1):239-43. doi: 10.1042/bj2170239.
4
Identification of ecdysonoic acid and 20-hydroxyecdysonoic acid isolated from developing eggs of Schistocerca gregaria and pupae of Spodoptera littoralis.从沙漠蝗发育中的卵和埃及棉叶夜蛾蛹中分离出蜕皮甾酸和20-羟基蜕皮甾酸的鉴定。
Biochem J. 1983 Jul 1;213(1):261-5. doi: 10.1042/bj2130261.
5
Isolation and identification of three ecdysteroid conjugates with a C-20 hydroxy group in eggs of Locusta migratoria.飞蝗卵中三种含C-20羟基蜕皮甾体缀合物的分离与鉴定
Steroids. 1982 Nov;40(5):551-60. doi: 10.1016/0039-128x(82)90075-7.
6
Identification of ecdysone 22-long-chain fatty acyl esters in newly laid eggs of the cattle tick Boophilus microplus.微小牛蜱新产虫卵中蜕皮激素22 - 长链脂肪酰酯的鉴定
Biochem J. 1986 Nov 15;240(1):131-8. doi: 10.1042/bj2400131.
7
Mechanism of hydroxylation at C-2 during the biosynthesis of ecdysone in ovaries of the locust, Schistocerca gregaria.蝗虫（沙漠蝗）卵巢中蜕皮激素生物合成过程中C-2位羟基化的机制。
Biochem J. 1984 Feb 1;217(3):783-9. doi: 10.1042/bj2170783.
8
RNAi-mediated knockdown of Shade negatively affects ecdysone-20-hydroxylation in the desert locust, Schistocerca gregaria.RNAi 介导的 Shade 基因敲低会对沙漠蝗，Schistocerca gregaria 的蜕皮激素 20-羟化酶产生负面影响。
J Insect Physiol. 2012 Jul;58(7):890-6. doi: 10.1016/j.jinsphys.2012.03.013. Epub 2012 Mar 29.
9
Formation of the A/B cis ring junction of ecdysteroids in the locust, Schistocerca gregaria.飞蝗（沙漠蝗）中蜕皮甾体A/B顺式环连接的形成
Biochem J. 1981 Jan 15;194(1):53-62. doi: 10.1042/bj1940053.
10
The ecdysteroids from young embryonated eggs of the tobacco hornworm.烟草天蛾未成熟胚胎卵中的蜕皮甾体。
Steroids. 1980 Sep;36(3):321-36. doi: 10.1016/0039-128x(80)90006-9.

引用本文的文献

1
Genetic characterization of candidate ecdysteroid kinases in Drosophila melanogaster.黑腹果蝇候选蜕皮激素激酶的遗传特征。
G3 (Bethesda). 2024 Nov 6;14(11). doi: 10.1093/g3journal/jkae204.
2
Phylogenomics of the Ecdysteroid Kinase-like (EcKL) Gene Family in Insects Highlights Roles in Both Steroid Hormone Metabolism and Detoxification.昆虫蜕皮激素激酶样（EcKL）基因家族的系统发生基因组学研究突出了其在类固醇激素代谢和解毒中的作用。
Genome Biol Evol. 2024 Feb 1;16(2). doi: 10.1093/gbe/evae019.
3
Enzymes of ecdysteroid transformation and inactivation in the midgut of the cotton leafworm, Spodoptera littoralis: properties and developmental profiles.棉铃虫（Spodoptera littoralis）中肠内蜕皮甾体转化和失活的酶：特性及发育概况
Biochem J. 1995 Dec 1;312 ( Pt 2)(Pt 2):561-8. doi: 10.1042/bj3120561.
4
Isolation and identification of ecdysteroid phosphates and acetylecdysteroid phosphates from developing eggs of the locust, Schistocerca gregaria.从沙漠蝗（Schistocerca gregaria）发育中的卵中分离并鉴定蜕皮甾醇磷酸酯和乙酰蜕皮甾醇磷酸酯。
Biochem J. 1984 Jul 15;221(2):459-64. doi: 10.1042/bj2210459.
5
Isolation and identification of 3-acetylecdysone 2-phosphate, a metabolite of ecdysone, from developing eggs of Schistocerca gregaria.从沙漠蝗发育中的卵中分离并鉴定蜕皮激素的一种代谢产物——3-乙酰蜕皮激素2-磷酸酯。
Biochem J. 1984 Jan 1;217(1):239-43. doi: 10.1042/bj2170239.
6
Formation of apolar ecdysteroid conjugates by ovaries of the house cricket Acheta domesticus in vitro.家蟋蟀卵巢体外形成非极性蜕皮甾体缀合物。
Biochem J. 1988 May 15;252(1):95-103. doi: 10.1042/bj2520095.

本文引用的文献

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The use of [18O4]phosphoric acid in the quantitation of phosphate by gas-liquid chromatography-mass spectrometry analysis.[18O4]磷酸在通过气-液色谱-质谱分析定量磷酸盐中的应用。
Anal Biochem. 1980 Sep 15;107(2):324-31. doi: 10.1016/0003-2697(80)90391-7.
2
Structure and configuration of fructose 2,6-bisphosphate by 31P and 13C nuclear magnetic resonance.通过磷-31和碳-13核磁共振对果糖2,6-二磷酸的结构和构型进行研究
Eur J Biochem. 1981 Jul;117(2):325-7. doi: 10.1111/j.1432-1033.1981.tb06340.x.
3
Identification of ecdysonoic acid and 20-hydroxyecdysonoic acid isolated from developing eggs of Schistocerca gregaria and pupae of Spodoptera littoralis.从沙漠蝗发育中的卵和埃及棉叶夜蛾蛹中分离出蜕皮甾酸和20-羟基蜕皮甾酸的鉴定。
Biochem J. 1983 Jul 1;213(1):261-5. doi: 10.1042/bj2130261.
4
A reliable radiochromatographic assay technique for hepatic microsomal 16 alpha-hydroxylase activity towards oestrone 3-sulphate. Comparison between pigmented and non-pigmented mature guinea pigs.一种用于检测肝微粒体对硫酸雌酮3的16α-羟化酶活性的可靠放射色谱分析技术。有色和无色成年豚鼠之间的比较。
Biochem J. 1980 Oct 1;191(1):221-7. doi: 10.1042/bj1910221.
5
Regulation and properties of a sex-specific hydroxylase system in female rat liver microsomes active on steroid sulfates. I. General characteristics.雌性大鼠肝微粒体中对甾体硫酸盐有活性的性别特异性羟化酶系统的调节与特性。I. 一般特征。
J Biol Chem. 1974 Mar 25;249(6):1940-5.
6
Structure and function of prothoracic glands and oenocytes in embryos and last larval instars of Oncopeltus fasciatus Dallas (Insecta, Heteroptera).豆长蝽胚胎及末龄幼虫前胸腺和绛色细胞的结构与功能（昆虫纲，异翅亚目）
Cell Tissue Res. 1976 Aug 26;171(3):331-50. doi: 10.1007/BF00224658.
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[Variations in the level of molting hormone during the second cycle of embryonic life in cockroaches].
C R Acad Hebd Seances Acad Sci D. 1976 Sep 27;283(6):647-50.
8
The follicle cell epithelium of maturing ovaries of Locusta migratoria: a new biosynthetic tissue for ecdysone.飞蝗成熟卵巢的卵泡细胞上皮：一种新的蜕皮激素生物合成组织。
Hoppe Seylers Z Physiol Chem. 1978 Oct;359(10):1427-34. doi: 10.1515/bchm2.1978.359.2.1427.
9
Carbon-13 nuclear-magnetic-resonance spectra of adenine cyclonucleosides and their phosphates. Effects of neighboring groups for elucidation of fine structure of nucleosides and nucleotides.
Eur J Biochem. 1978 Sep 15;90(1):205-12. doi: 10.1111/j.1432-1033.1978.tb12592.x.
10
Specific metabolic pathways of steroid sulfates in human liver microsomes.人类肝脏微粒体中甾体硫酸盐的特定代谢途径。
J Clin Endocrinol Metab. 1976 Jul;43(1):56-63. doi: 10.1210/jcem-43-1-56.

从沙漠蝗虫（Schistocerca gregaria）新产的卵中鉴定蜕皮激素、2-脱氧蜕皮激素、20-羟基蜕皮激素和2-脱氧-20-羟基蜕皮激素的22-磷酸酯。

Identification of the 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone from newly laid eggs of the desert locust, Schistocerca gregaria.

作者信息

Isaac R E, Rose M E, Rees H H, Goodwin T W

出版信息

Biochem J. 1983 Aug 1;213(2):533-41. doi: 10.1042/bj2130533.

DOI:10.1042/bj2130533
PMID:6684423
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC1152159/
Abstract

The four major ecdysteroid (insect moulting hormone) conjugates present in the newly laid eggs of the desert locust, Schistocera gregaria, have been purified by reversed-phase and anion-exchange high-performance liquid chromatography. The steroid moieties were identified as ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone. Phosphate analysis of acid-hydrolysed samples showed a steroid:phosphate ratio of approx. 1:1 for all four compounds. The intact conjugates were identified as ecdysone 22-phosphate, 2-deoxyecdysone 22-phosphate, 20-hydroxyecdysone 22-phosphate and 2-deoxy-20-hydroxyecdysone 22-phosphate by fast atom bombardment mass spectrometry and 1H, 13C and 31P n.m.r. The significance of ecdysteroid phosphates as a source of free hormone during embryogenesis is discussed.

摘要

通过反相和阴离子交换高效液相色谱法，已对沙漠蝗虫（Schistocera gregaria）新产卵中存在的四种主要蜕皮甾类（昆虫蜕皮激素）缀合物进行了纯化。甾体部分被鉴定为蜕皮酮、2-脱氧蜕皮酮、20-羟基蜕皮酮和2-脱氧-20-羟基蜕皮酮。酸水解样品的磷酸盐分析表明，所有四种化合物的甾体：磷酸盐比例约为1:1。通过快原子轰击质谱法以及1H、13C和31P核磁共振，完整的缀合物被鉴定为蜕皮酮22-磷酸酯、2-脱氧蜕皮酮22-磷酸酯、20-羟基蜕皮酮22-磷酸酯和2-脱氧-20-羟基蜕皮酮22-磷酸酯。本文讨论了蜕皮甾类磷酸盐作为胚胎发育过程中游离激素来源的重要性。