从沙漠蝗虫（Schistocerca gregaria）新产的卵中鉴定蜕皮激素、2-脱氧蜕皮激素、20-羟基蜕皮激素和2-脱氧-20-羟基蜕皮激素的22-磷酸酯。
Identification of the 22-phosphate esters of ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone from newly laid eggs of the desert locust, Schistocerca gregaria.
作者信息
Isaac R E, Rose M E, Rees H H, Goodwin T W
出版信息
Biochem J. 1983 Aug 1;213(2):533-41. doi: 10.1042/bj2130533.
Abstract
The four major ecdysteroid (insect moulting hormone) conjugates present in the newly laid eggs of the desert locust, Schistocera gregaria, have been purified by reversed-phase and anion-exchange high-performance liquid chromatography. The steroid moieties were identified as ecdysone, 2-deoxyecdysone, 20-hydroxyecdysone and 2-deoxy-20-hydroxyecdysone. Phosphate analysis of acid-hydrolysed samples showed a steroid:phosphate ratio of approx. 1:1 for all four compounds. The intact conjugates were identified as ecdysone 22-phosphate, 2-deoxyecdysone 22-phosphate, 20-hydroxyecdysone 22-phosphate and 2-deoxy-20-hydroxyecdysone 22-phosphate by fast atom bombardment mass spectrometry and 1H, 13C and 31P n.m.r. The significance of ecdysteroid phosphates as a source of free hormone during embryogenesis is discussed.
摘要
通过反相和阴离子交换高效液相色谱法，已对沙漠蝗虫（Schistocera gregaria）新产卵中存在的四种主要蜕皮甾类（昆虫蜕皮激素）缀合物进行了纯化。甾体部分被鉴定为蜕皮酮、2-脱氧蜕皮酮、20-羟基蜕皮酮和2-脱氧-20-羟基蜕皮酮。酸水解样品的磷酸盐分析表明，所有四种化合物的甾体：磷酸盐比例约为1:1。通过快原子轰击质谱法以及1H、13C和31P核磁共振，完整的缀合物被鉴定为蜕皮酮22-磷酸酯、2-脱氧蜕皮酮22-磷酸酯、20-羟基蜕皮酮22-磷酸酯和2-脱氧-20-羟基蜕皮酮22-磷酸酯。本文讨论了蜕皮甾类磷酸盐作为胚胎发育过程中游离激素来源的重要性。
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