Department of Radiology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
Department of Pathology, Renmin Hospital of Wuhan University, Wuhan 430060, Hubei, China.
Contrast Media Mol Imaging. 2021 Jul 10;2021:4799116. doi: 10.1155/2021/4799116. eCollection 2021.
This work explored the diagnostic value of different subtypes of meningiomas under T2WI low signal based on analysis of variance (ANOVA), and the expression differences of Ki67, VEGF, and P73 in different subtypes were analyzed. 67 patients with meningioma confirmed surgically and pathologically in hospital were selected as the research subjects, whose pathological classification occurs with obvious low signal on T2WI. First, the age distribution of the subjects and the distribution of different subtypes were counted. Then, ANOVA was adopted to analyze the MRI imaging signs of patients with different subtypes of meningioma. Finally, the differences of Ki67, VEGF, and P73 proteins and mRNA expression levels in different subtypes were detected via immunohistochemical assay and qPCR. The results showed that the proportion of patients with transitional meningioma was the most, which was 43.28%, while the proportion of patients with meningeal melanoma was the least, which was 7.46%. In patients with transitional meningioma, the MRI images showed mixed signals in different layers. Fibrous MRI images showed hyalinosis and calcification of collagen fibers in the tumor, with low T2WI signal. Sand-shape MRI images showed double low signals. MRI images of meningeal melanoma showed high signal on T1-weighted Imaging (T1WI) and low signal on T2WI. The protein expression and mRNA levels of Ki67 and P73 in transitional meningioma were evidently higher in contrast to those in fibrous meningioma ( < 0.05). The expression level of VEGF protein and mRNA in meningeal melanoma were notably higher in contrast to those in fibro meningioma ( < 0.05). It was revealed that the MRI images of the four subtypes of meningiomas under ANOVA-based T2WI low signal were quite different, and the expressions of Ki67, P73, and VEGF in different subtypes had significant differences. This work provided a reference basis for the preoperative diagnosis, treatment, and prognosis of meningiomas.
本研究基于方差分析（ANOVA）探讨 T2WI 低信号下不同脑膜瘤亚型的诊断价值，并分析不同亚型中 Ki67、VEGF 和 P73 的表达差异。选取我院经手术病理证实的脑膜瘤患者 67 例，病理分型 T2WI 呈明显低信号。首先统计患者的年龄分布及不同亚型的分布情况，然后采用 ANOVA 分析不同亚型脑膜瘤患者的 MRI 影像学征象，最后通过免疫组化法和 qPCR 检测不同亚型中 Ki67、VEGF 和 P73 蛋白及其 mRNA 表达水平的差异。结果显示，过渡型脑膜瘤患者比例最多，为 43.28%，脑膜黑色素瘤患者比例最少，为 7.46%。过渡型脑膜瘤患者 MRI 图像表现为不同层次的混合信号，纤维型 MRI 图像显示肿瘤内胶原纤维玻璃样变和钙化，T2WI 呈低信号；砂粒型 MRI 图像呈双低信号；脑膜黑色素瘤 MRI 图像 T1WI 呈高信号，T2WI 呈低信号。与纤维型脑膜瘤相比，过渡型脑膜瘤中 Ki67 和 P73 的蛋白表达和 mRNA 水平明显升高（<0.05）。脑膜黑色素瘤中 VEGF 蛋白和 mRNA 的表达水平明显高于纤维型脑膜瘤（<0.05）。结果表明，基于 ANOVA 的 T2WI 低信号下四种脑膜瘤亚型的 MRI 图像有明显差异，不同亚型中 Ki67、P73 和 VEGF 的表达有显著差异。本研究为脑膜瘤的术前诊断、治疗和预后提供了参考依据。
