Zhang Meng, Zhou Junjie, Jiao Li, Xu Longjiang, Hou Lin, Yin Bin, Qiang Boqin, Lu Shuaiyao, Shu Pengcheng, Peng Xiaozhong
Institute of Medical Biology, Chinese Academy of Medical Sciences, and Peking Union Medical College, Kunming, China.
The State Key Laboratory of Medical Molecular Biology, Department of Molecular Biology and Biochemistry, Institute of Basic Medical Sciences, Chinese Academy of Medical Sciences and Peking Union Medical College, Beijing, China.
Front Neurosci. 2021 Jul 20;15:709684. doi: 10.3389/fnins.2021.709684. eCollection 2021.
Neurogenesis is a complex process that depends on the delicate regulation of spatial and temporal gene expression. In our previous study, we found that transcribed ultra-conserved regions (T-UCRs), a class of long non-coding RNAs that contain UCRs, are expressed in the developing nervous systems of mice, rhesus monkeys, and humans. In this study, we first detected the full-length sequence of T-uc.189, revealing that it was mainly concentrated in the ventricular zone (VZ) and that its expression decreased as the brain matured. Moreover, we demonstrated that knockdown of T-uc.189 inhibited neurogenesis. In addition, we found that T-uc.189 positively regulated the expression of serine-arginine-rich splicing factor 3 (). Taken together, our results are the first to demonstrate that T-uc.189 regulates the expression of to maintain normal neurogenesis during cortical development.
神经发生是一个复杂的过程，它依赖于空间和时间基因表达的精细调控。在我们之前的研究中，我们发现转录的超保守区域（T-UCRs），一类包含超保守区域的长链非编码RNA，在小鼠、恒河猴和人类的发育中的神经系统中表达。在本研究中，我们首先检测了T-uc.189的全长序列，发现它主要集中在脑室区（VZ），并且其表达随着大脑成熟而降低。此外，我们证明敲低T-uc.189会抑制神经发生。另外，我们发现T-uc.189正向调节富含丝氨酸-精氨酸的剪接因子3（）的表达。综上所述，我们的结果首次证明T-uc.189在皮质发育过程中调节的表达以维持正常的神经发生。 （注：原文中“serine-arginine-rich splicing factor 3 ()”括号内缺失具体内容）
