Howarth J V, Millar N C, Gutfreund H
Marine Biological Association, Plymouth, U.K.
Biochem J. 1987 Dec 15;248(3):677-82. doi: 10.1042/bj2480677.
A rapid-response stopped-flow calorimeter for small samples of reagents is described. The construction, performance characteristics and operational limitations are described, along with an example of its ability to resolve the kinetics of an enzyme-catalysed hydrolysis. It is thought likely that the method would find useful application in a variety of chemical and biochemical investigations.
本文描述了一种用于少量试剂样品的快速响应停流热量计。文中介绍了该热量计的构造、性能特点和操作局限性,并给出了一个示例,展示了其解析酶催化水解动力学的能力。据信,该方法可能会在各种化学和生化研究中得到有用的应用。