Orzabal Marcus R, Naik Vishal D, Lee Jehoon, Wu Guoyao, Ramadoss Jayanth
Department of Veterinary Physiology and Pharmacology, College of Veterinary Medicine and Biomedical Sciences, Texas A&M University, College Station, TX, USA.
Department of Animal Science, College of Agriculture and Life Sciences, Texas A&M University, College Station, TX, USA.
Metabol Open. 2021 Jul 16;11:100107. doi: 10.1016/j.metop.2021.100107. eCollection 2021 Sep.
Electronic cigarettes (e-cigs) are a form of tobacco product that has become increasingly popular over the past decade. Despite the known health consequences of tobacco product exposure during pregnancy, a substantial number of daily smokers will continue to smoke during pregnancy. Our current knowledge on the effects of e-cig aerosol exposure during pregnancy is limited to a small number of animal studies, which have identified several e-cig aerosol-induced disruptions to the physiology of normal development.
To further assess the impact of prenatal e-cig aerosol exposure on maternal and fetal health, we examined the amino acid signature profiles in maternal and fetal plasma, as well as in the fetal lungs, a sensitive target organ for prenatal tobacco product exposure. Pregnant Sprague Dawley rats were randomly assigned to one of three groups and were exposed to either e-cig aerosols containing nicotine, e-cig aerosols without nicotine, or room air. Dams were exposed utilizing a state-of-the-art custom engineered e-cig vaping system that is compatible with commercially available e-cig atomizers and enables a translational inhalation delivery method comparable to human vaping.
We determined that gestational exposure to e-cig aerosols results in significant alterations to the amino acid profile in the maternal and fetal compartments, including the fetal lungs. The data shows a targeted disruption to the nitric oxide pathway, branched-chain amino acid metabolism, fetal protein synthesis, and urea cycle.
The data presented herein provides additional support that gestational e-cig aerosol exposure can impact crucial biological processes and exemplifies the need for extensive research on exposure to e-cig aerosols.
电子烟是一种烟草制品，在过去十年中越来越受欢迎。尽管已知孕期接触烟草制品会对健康产生影响，但仍有相当数量的每日吸烟者在孕期会继续吸烟。我们目前对孕期接触电子烟烟雾影响的了解仅限于少数动物研究，这些研究已确定电子烟烟雾会对正常发育的生理过程造成多种干扰。
为了进一步评估产前接触电子烟烟雾对母婴健康的影响，我们检测了母体和胎儿血浆以及胎儿肺部（产前烟草制品接触的敏感靶器官）中的氨基酸特征谱。将怀孕的斯普拉格-道利大鼠随机分为三组，分别暴露于含尼古丁的电子烟烟雾、不含尼古丁的电子烟烟雾或室内空气中。利用一种先进的定制工程电子烟雾化系统对母鼠进行暴露，该系统与市售电子烟雾化器兼容，并能实现与人类吸电子烟相当的转化吸入给药方式。
我们确定孕期接触电子烟烟雾会导致母体和胎儿各部分，包括胎儿肺部的氨基酸谱发生显著改变。数据显示对一氧化氮途径、支链氨基酸代谢、胎儿蛋白质合成和尿素循环有针对性的干扰。
本文提供的数据进一步支持了孕期接触电子烟烟雾会影响关键生物学过程的观点，并例证了对接触电子烟烟雾进行广泛研究的必要性。
