Rose H, Schnitzler N, Kammermeier H
Dept. of Physiology, Medical Faculty, RHTH Aachen.
Biomed Biochim Acta. 1987;46(8-9):S622-7.
Progress in the utilisation of the model "isolated cardiac myocytes" was achieved by designing a special stimulation chamber. This chamber allows eliciting of contraction of suspended myocytes by electrical stimulation. Oxygen consumption was thereby linearly enhanced dependent on the rate of stimulation. Different extent of shortening was observed indicating different inotropic state. Considering the myocytes to behave as a spring (with small delta L) a correlate to work performed was obtained which allowed evaluation of changes in ATP/O-ratios. Uptake of 3-O-methyl-D-glucose was dependent on the mechanical and therefore metabolic activity of the cells. The results show that transfer of glucose across the myocardial sarcolemma is adapted to the metabolic demand by affinity variation and not by recruitment of the transporters.
通过设计一种特殊的刺激室，“分离心肌细胞”模型的应用取得了进展。该室可通过电刺激引发悬浮心肌细胞的收缩。由此，氧气消耗随刺激频率呈线性增加。观察到不同程度的缩短，表明存在不同的变力状态。将心肌细胞视为一个弹簧（ΔL较小），可得出与所做功的相关性，从而能够评估ATP/O比值的变化。3 - O - 甲基 - D - 葡萄糖的摄取取决于细胞的机械活性以及代谢活性。结果表明，葡萄糖跨心肌肌膜的转运是通过亲和力变化而非转运体的募集来适应代谢需求的。
