Rose H, Kammermeier H
Pflugers Arch. 1986 Jul;407(1):116-8. doi: 10.1007/BF00580731.
An experimental setup has been developed, which allows electrical stimulation of cardiac myocytes and simultaneous measurement of oxygen consumption, lactate production, extent of shortening and of substrate uptake. In resting cells and in cells stimulated with 120 to 480/min the oxygen consumption ranged from 25 to 100 microliter/min X gww, with a linear relationship between rate of stimulation and oxygen consumption (VO2). When using 5 mM glucose plus 2 mM pyruvate or 10 mM lactate as substrates, isoproterenol (8 X 10(-8) M) augmented contraction and VO2 at all rates of stimulation. Assuming Hook's law for passive elastic behavior for the contracting myocytes over the length change observed, a good correlation exists between the degree of cell shortening calculated from VO2 per beat and the degree of contraction measured. This correlation can be used as a measure of the economy of O2 utilization.
已开发出一种实验装置，该装置可对心肌细胞进行电刺激，并同时测量耗氧量、乳酸生成量、缩短程度和底物摄取量。在静息细胞以及以每分钟120至480次进行刺激的细胞中，耗氧量范围为每分钟25至100微升/克湿重，刺激速率与耗氧量（VO₂）之间呈线性关系。当使用5毫摩尔/升葡萄糖加2毫摩尔/升丙酮酸或10毫摩尔/升乳酸作为底物时，异丙肾上腺素（8×10⁻⁸摩尔/升）在所有刺激速率下均增强收缩和VO₂。假设在观察到的长度变化范围内收缩心肌细胞的被动弹性行为符合胡克定律，则根据每搏VO₂计算出的细胞缩短程度与测量的收缩程度之间存在良好的相关性。这种相关性可作为氧气利用效率的一种衡量指标。
