评估人类单个免疫细胞中 X 染色体失活和基因表达的逃逸。

Assessment of escape from X chromosome inactivation and gene expression in single human immune cells.

机构信息

Research Department Virus Immunology, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg 20251, Germany.

Technology Platform Flow Cytometry / FACS, Heinrich Pette Institute, Leibniz Institute for Experimental Virology, Martinistrasse 52, Hamburg 20251, Germany.

出版信息

STAR Protoc. 2021 Jul 20;2(3):100641. doi: 10.1016/j.xpro.2021.100641. eCollection 2021 Sep 17.

DOI:10.1016/j.xpro.2021.100641

PMID:34355200

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8319808/

Abstract

X-chromosomal genes escaping from X chromosome inactivation (XCI) in immune cells can contribute to sex-specific differences in immune responses. This protocol describes the specific steps to determine escape from XCI and to simultaneously quantify mRNA expression of multiple genes at the single immune cell level using a single-nucleotide polymorphism approach. The protocol furthermore allows the analysis of allele-specific expression of X-chromosomal genes. For complete details on the use and execution of this protocol, please refer to Hagen et al. (2020).

摘要

X 染色体上的基因逃避 X 染色体失活（XCI）可导致免疫反应的性别特异性差异。本方案描述了使用单核苷酸多态性方法确定 XCI 逃逸并同时定量单个免疫细胞中多个基因的 mRNA 表达的具体步骤。该方案还允许分析 X 染色体基因的等位基因特异性表达。有关此方案使用和执行的完整详细信息，请参阅 Hagen 等人。（2020）。