Allen Institute for Immunology, 615 Westlake Avenue N, Seattle, WA 98109, USA.
STAR Protoc. 2021 Nov 5;2(4):100900. doi: 10.1016/j.xpro.2021.100900. eCollection 2021 Dec 17.
Deep immune profiling is essential for understanding the human immune system in health and disease. Successful biological interpretation of this data requires consistent laboratory processing with minimal batch-to-batch variation. Here, we detail a robust pipeline for the profiling of human peripheral blood mononuclear cells by both high-dimensional flow cytometry and single-cell RNA-seq. These protocols reduce batch effects, generate reproducible data, and increase throughput. For complete details on the use and execution of this protocol, please refer to Savage et al. (2021).
深度免疫分析对于了解健康和疾病中的人体免疫系统至关重要。要成功地对这些数据进行生物学解释，就需要用最小的批间变化进行一致的实验室处理。在此，我们详细介绍了一种通过高维流式细胞术和单细胞 RNA 测序对人外周血单核细胞进行分析的稳健方案。这些方案减少了批次效应，生成了可重复的数据，并提高了通量。有关此方案的使用和执行的完整详细信息，请参阅 Savage 等人（2021 年）。
