Bostancioglu K, Mecham R P, Wallach J M
UA CNRS 244, I.C.B.M.C., Université Lyon I, Villeurbanne, France.
Biochem Int. 1987 Aug;15(2):263-9.
Rate of elastolysis by pancreatic and leukocyte elastases of normal and copper deficient porcine aortic elastins were measured using a conductimetric method. Kinetics obey to Michaelis-Menten model for both substrates and enzymes. KM and Vmax values derived from Lineweaver-Burk plots indicate that, if a near uniformity exists in KM, differences were observed in catalytic rates, kcat increasing approximately 40% for copper deficient elastin elastolysis by leukocyte elastase. This higher susceptibility to proteolysis may have implications for understanding turnover of elastin in tissues.
采用电导法测定了正常和缺铜猪主动脉弹性蛋白被胰弹性蛋白酶和白细胞弹性蛋白酶分解的速率。底物和酶的动力学均符合米氏模型。从Lineweaver-Burk图得出的KM和Vmax值表明，如果KM值存在近乎一致性，那么在催化速率上会观察到差异，白细胞弹性蛋白酶对缺铜弹性蛋白的分解作用中，kcat增加了约40%。这种对蛋白水解更高的敏感性可能对理解组织中弹性蛋白的更新有影响。
