人白细胞弹性蛋白酶和猪胰弹性蛋白酶的弹性溶解作用比较。

Comparison of the elastolytic effects of human leukocyte elastase and porcine pancreatic elastase.

作者信息

Senior R M, Bielefeld D R, Starcher B C

出版信息

Adv Exp Med Biol. 1977;79:249-61. doi: 10.1007/978-1-4684-9093-0_21.

Abstract

Porcine and bovine elastins were digested by human leukocyte elastase and porcine pancreatic elastase. The enzymes showed similarities in the extent to which they digested elastin and the pattern and quantitative distribution of N-terminal amino acids in the digests. However, fingerprints of the digests showed differences between the products of leukocyte elastase and pancreatic elastase. Each enzyme produced its characteristic fingerprint irrespective of whether the elastin substrate was obtained from ligament, pleura or lung parenchyma. The enzymes also digested tropoelastin differently. The results suggest that leukocyte elastase and pancreatic elastase should not be considered interchangeable in experimental models of tissue injury.

摘要

人白细胞弹性蛋白酶和猪胰弹性蛋白酶对猪和牛的弹性蛋白进行了消化。这些酶在消化弹性蛋白的程度、消化产物中N端氨基酸的模式和定量分布方面表现出相似性。然而，消化产物的指纹图谱显示白细胞弹性蛋白酶和胰弹性蛋白酶的产物之间存在差异。无论弹性蛋白底物是从韧带、胸膜还是肺实质获得的，每种酶都产生其特征性指纹图谱。这些酶对原弹性蛋白的消化方式也不同。结果表明，在组织损伤的实验模型中，白细胞弹性蛋白酶和胰弹性蛋白酶不应被视为可互换的。