Koshizaka T, Nishikimi M, Tanaka M, Nakashima K, Ozawa T, Yagi K
Institute of Applied Biochemistry, Yagi Memorial Park, Gifu, Japan.
Biochem Int. 1987 Oct;15(4):779-83.
In vitro synthesis of L-gulono-gamma-lactone oxidase [L-gulono-gamma-lactone:oxygen 2-oxidoreductase, EC 1.1.3.8], one of the microsomal flavin enzymes, was performed with poly(A)+ RNA of rat liver using the rabbit reticulocyte lysate system in order to study the biosynthesis of the enzyme. The apparent molecular weight of the synthesized enzyme protein was almost the same as that of the purified L-gulono-gamma-lactone oxidase from rat liver. It was demonstrated that the enzyme protein was not detectable when guinea pig poly(A)+ RNA was used for the translation, indicating that the mRNA for the enzyme is absent in the guinea pig or, if it exists, is not translatable.
为了研究微粒体黄素酶之一的L-古洛糖酸-γ-内酯氧化酶[L-古洛糖酸-γ-内酯：氧2-氧化还原酶，EC 1.1.3.8]的生物合成，利用兔网织红细胞裂解物系统，以大鼠肝脏的聚腺苷酸加尾RNA（poly(A)+ RNA）进行了体外合成。合成的酶蛋白的表观分子量与从大鼠肝脏纯化的L-古洛糖酸-γ-内酯氧化酶几乎相同。结果表明，当使用豚鼠的聚腺苷酸加尾RNA进行翻译时，无法检测到该酶蛋白，这表明豚鼠体内不存在该酶的信使核糖核酸（mRNA），或者即便存在，也无法进行翻译。
