一种微电子传导生物传感器。

A microelectronic conductimetric biosensor.

作者信息

Watson L D, Maynard P, Cullen D C, Sethi R S, Brettle J, Lowe C R

机构信息

Biotechnology Centre, University of Cambridge, Great Britain.

出版信息

Biosensors. 1987;3(2):101-15. doi: 10.1016/s0265-928x(87)80003-2.

DOI:10.1016/s0265-928x(87)80003-2

PMID:3435561

Abstract

The fabrication and operation of a microelectronic conductimetric biosensor is described. The device monitors the change in solution conductance occasioned by the catalytic action of enzymes immobilised over a planar conductance cell comprising serpentined and interdigitated metal conductor tracks. The output of the instrument was linear over a 3 min period on addition of urea to a sample cell overlaid with immobilised urease. The responses to any given urea concentration were reproducible to within approximately +/- 1%. The device responds to urea present in serum samples.

摘要

描述了一种微电子电导生物传感器的制造和操作。该装置监测由固定在包含蜿蜒和叉指状金属导体轨道的平面电导池上的酶的催化作用引起的溶液电导变化。在向覆盖有固定化脲酶的样品池中添加尿素后，仪器的输出在3分钟内呈线性。对任何给定尿素浓度的响应重复性在约±1%以内。该装置对血清样品中存在的尿素有响应。