尿素生物传感器的实时动态流体测量的制作。

Cooperative Course of Nano-Medical Device Engineering, Hallym University, Chuncheon 24252, Korea.

Department of Materials Science and Engineering, Hallym University, Chuncheon 24252, Korea.

Sensors (Basel). 2018 Aug 9;18(8):2607. doi: 10.3390/s18082607.

In this study, a portable urea sensor that monitors the urea concentration in flow conditions was fabricated. We propose an electrochemical sensor that continually measures the urea concentration of samples flowing through it at a constant flow rate in real time. For the electrochemical sensing, a porous silk fibroin membrane with immobilized urease was mounted in a polydimethylsiloxane (PDMS) sensor housing. The fabricated urea sensor elicited linear current⁻concentration characteristics in the clinically significant concentration range (0.1⁻20 mM) based on peritoneal dialysis. The sensor maintained the linear current⁻concentration characteristics during operation in flow conditions.

在这项研究中，我们制备了一种可在流动条件下监测尿素浓度的便携式尿素传感器。我们提出了一种电化学传感器，可在恒定流速下实时连续测量流过它的样品的尿素浓度。对于电化学传感，将固定有脲酶的多孔丝素膜安装在聚二甲基硅氧烷（PDMS）传感器外壳中。基于腹膜透析，所制备的尿素传感器在临床相关浓度范围内（0.1⁻20 mM）产生了线性电流⁻浓度特征。该传感器在流动条件下运行时保持了线性电流⁻浓度特征。