苯并[a]芘-珠蛋白加合物的研究。
Investigation of benzo[a]pyrene-globin adducts.
作者信息
Wallin H, Jeffrey A M, Santella R M
出版信息
Cancer Lett. 1987 May;35(2):139-46. doi: 10.1016/0304-3835(87)90037-1.
The nature of the adducts formed between benzo[a]pyrene (BP) and globin were investigated in animals treated with [3H]BP. Modification levels on globin were determined by radioactivity measurements. Since BP tetraols can be released from benzo[a]pyrene diol epoxide modified protein and DNA by acid treatment, globin samples were treated with acid, released tetraols separated by HPLC and quantitated by scintillation counting. In addition, acid released material was measured in a competitive enzyme linked immunosorbent assay (ELISA) using antibodies which recognize BP tetraols. Both measurements indicate that only 2% of bound radioactivity could be released as free BP tetraols. These studies indicate that benzo[a]pyrene diol epoxide may not be the major metabolite of BP involved in globin binding.
在接受[³H]苯并[a]芘（BP）处理的动物中，研究了苯并[a]芘（BP）与珠蛋白形成的加合物的性质。通过放射性测量确定珠蛋白上的修饰水平。由于BP四醇可通过酸处理从苯并[a]芘二醇环氧化物修饰的蛋白质和DNA中释放出来，因此对珠蛋白样品进行酸处理，通过高效液相色谱法分离释放出的四醇，并通过闪烁计数进行定量。此外，使用识别BP四醇的抗体，通过竞争性酶联免疫吸附测定（ELISA）测量酸释放物质。两种测量均表明，只有2%的结合放射性可以作为游离BP四醇释放出来。这些研究表明，苯并[a]芘二醇环氧化物可能不是参与珠蛋白结合的BP的主要代谢产物。
Zotero 插件