Mottola Filomena, Santonastaso Marianna, Iovine Concetta, Rossetti Cristina, Ronga Valentina, Rocco Lucia
Department of Environmental, Biological and Pharmaceutical Sciences and Technologies, University of Campania Luigi Vanvitelli, 81100 Caserta, Italy.
Department of Woman, Child and General and Special Surgery, University of Campania, Luigi Vanvitelli, 80138 Napoli, Italy.
Antioxidants (Basel). 2021 Jul 17;10(7):1137. doi: 10.3390/antiox10071137.
Oxidative imbalances in the gestational phase are responsible for certain complications during pregnancy and for foetal and neonatal genetic disorders. In this work, using human amniocytes, we aimed to evaluate the protection provided to foetal DNA by two concentrations of antioxidant molecules, α-lipoic acid (LA) and curcumin (Cur), against hydrogen peroxide (HO)-induced damage. Genotoxicity tests, performed by the random amplification of polymorphic DNA (RAPD-PCR) technique and TUNEL tests, showed that the lowest concentration of LA-protected cells and DNA from HO insults. However, a greater ability to protect the amniocytes' DNA against HO was observed following co-treatment with the highest concentration of Cur with HO. In fact, a genomic template stability (GTS%) similar to that of the negative control and a statistically significant reduction in the DNA fragmentation index (DFI) were revealed. Moreover, following a combined treatment with both antioxidants and HO, no statistical difference from controls was observed, in terms of both induced mutations and DNA breaks. Furthermore, no effect on morphology or cell viability was observed. The results demonstrate the ability of LA and Cur to protect the genetic material of amniocytes against genotoxic insults, suggesting their beneficial effects in pathologies related to oxidative stress.
妊娠期的氧化失衡是孕期某些并发症以及胎儿和新生儿遗传疾病的原因。在这项研究中，我们使用人羊膜细胞，旨在评估两种浓度的抗氧化分子α-硫辛酸（LA）和姜黄素（Cur）对胎儿DNA免受过氧化氢（HO）诱导损伤的保护作用。通过随机扩增多态性DNA（RAPD-PCR）技术和TUNEL试验进行的遗传毒性测试表明，最低浓度的LA可保护细胞和DNA免受HO损伤。然而，在与最高浓度的Cur和HO共同处理后，观察到对羊膜细胞DNA免受HO损伤的能力更强。事实上，揭示了与阴性对照相似的基因组模板稳定性（GTS%）以及DNA片段化指数（DFI）的统计学显著降低。此外，在两种抗氧化剂与HO联合处理后，在诱导突变和DNA断裂方面均未观察到与对照的统计学差异。此外，未观察到对形态或细胞活力的影响。结果证明了LA和Cur保护羊膜细胞遗传物质免受遗传毒性损伤的能力，表明它们在与氧化应激相关的病理学中具有有益作用。
