孤啡肽增加糖尿病大鼠肾脏、肝脏和大脑中的抗氧化剂表达。

Nociceptin Increases Antioxidant Expression in the Kidney, Liver and Brain of Diabetic Rats.

作者信息

Adeghate Ernest, D'Souza Crystal M, Saeed Zulqarnain, Al Jaberi Saeeda, Tariq Saeed, Kalász Huba, Tekes Kornélia, Adeghate Ernest A

机构信息

Department of Pharmacodynamics, Semmelweis University, Nagyvárad Tér 4, H-1089 Budapest, Hungary.

Department of Anatomy, College of Medicine & Health Sciences, United Arab Emirates University, Al Ain P.O. Box 17666, United Arab Emirates.

出版信息

Biology (Basel). 2021 Jul 3;10(7):621. doi: 10.3390/biology10070621.

DOI:10.3390/biology10070621

PMID:34356475

原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8301093/

Abstract

Nociceptin (NC) consists of 17 amino acids (aa) and takes part in the processing of learning and memory. The role of NC in the induction of endogenous antioxidants in still unclear. We examined the effect of NC on the expression of endogenous antioxidants in kidney, liver, cerebral cortex (CC), and hippocampus after the onset of diabetes mellitus, using enzyme-linked immunosorbent assay and immunohistochemistry. Exogenous NC (aa chain 1-17; 10 µg/kg body weight) was given intraperitoneally to normal and diabetic rats for 5 days. Our results showed that catalase (CAT) is present in the proximal (PCT) and distal (DCT) convoluted tubules of kidney, hepatocytes, and neurons of CC and hippocampus. The expression of CAT was significantly ( < 0.05) reduced in the kidney of normal and diabetic rats after treatment with NC. However, NC markedly ( < 0.001) increased the expression CAT in the liver and neurons of CC of diabetic rats. Superoxide dismutase (SOD) is widely distributed in the PCT and DCT of kidney, hepatocytes, and neurons of CC and hippocampus. NC significantly ( < 0.001) increased the expression of SOD in hepatocytes and neurons of CC and the hippocampus but not in the kidney. Glutathione reductase (GRED) was observed in kidney tubules, hepatocytes and neurons of the brain. NC markedly increased ( < 0.001) the expression of GRED in PCT and DCT cells of the kidney and hepatocytes of liver and neurons of CC. In conclusion, NC is a strong inducer of CAT, SOD, and GRED expression in the kidney, liver and brain of diabetic rats.

摘要

孤啡肽（NC）由17个氨基酸组成，参与学习和记忆过程。NC在诱导内源性抗氧化剂方面的作用尚不清楚。我们使用酶联免疫吸附测定法和免疫组织化学方法，研究了糖尿病发病后NC对肾脏、肝脏、大脑皮层（CC）和海马体中内源性抗氧化剂表达的影响。将外源性NC（氨基酸链1 - 17；10μg/kg体重）腹腔注射给正常和糖尿病大鼠，持续5天。我们的结果表明，过氧化氢酶（CAT）存在于肾脏的近端（PCT）和远端（DCT）曲管、肝细胞以及CC和海马体的神经元中。用NC处理后，正常和糖尿病大鼠肾脏中CAT的表达显著降低（<0.05）。然而，NC显著（<0.001）增加了糖尿病大鼠肝脏和CC神经元中CAT的表达。超氧化物歧化酶（SOD）广泛分布于肾脏的PCT和DCT、肝细胞以及CC和海马体的神经元中。NC显著（<0.001）增加了CC和海马体的肝细胞和神经元中SOD的表达，但在肾脏中未增加。在肾小管、肝细胞和脑神经元中观察到谷胱甘肽还原酶（GRED）。NC显著增加（<0.001）了肾脏PCT和DCT细胞、肝脏肝细胞以及CC神经元中GRED的表达。总之，NC是糖尿病大鼠肾脏、肝脏和大脑中CAT、SOD和GRED表达的强力诱导剂。