维生素D充足的断奶豚鼠肾脏25-羟胆钙化醇1α-羟化酶的亚线粒体定位

Submitochondrial localization of kidney 25-hydroxycholecalciferol 1 alpha-hydroxylase in vitamin D repleted weanling guinea pigs.

作者信息

Yan X, Charette G, Delvin E E

机构信息

Genetics Unit, Shriners Hospital, Montréal, Que., Canada.

出版信息

Biochem Cell Biol. 1987 Jul;65(7):673-6. doi: 10.1139/o87-089.

DOI:10.1139/o87-089

PMID:3435664

Abstract

We have studied the submitochondrial localization of guinea-pig kidney 25-hydroxycholecalciferol 1 alpha-hydroxylase. Treatment of the mitochondrial-enriched fraction with recrystallized digitonin produced mitoplasts bordered by a single membrane and with intact matrix. They contained nearly 90% of the 25-hydroxycholecalciferol 1 alpha-hydroxylase activity and nearly 100% of the cytochrome-c:oxygen oxidoreductase. Amine:oxygen oxidoreductase activity remained mainly in the outer membrane fraction. These data show that 25-hydroxycholecalciferol 1 alpha-hydroxylase has a distribution similar to that of steroid hydroxylases.

摘要

我们研究了豚鼠肾脏25-羟基胆钙化醇1α-羟化酶的亚线粒体定位。用重结晶洋地黄皂苷处理富含线粒体的部分，产生了由单层膜界定且基质完整的线粒体小体。它们含有近90%的25-羟基胆钙化醇1α-羟化酶活性和近100%的细胞色素c：氧氧化还原酶。胺：氧氧化还原酶活性主要保留在外膜部分。这些数据表明，25-羟基胆钙化醇1α-羟化酶的分布与类固醇羟化酶相似。