College of Korean Medicine, Gachon University, Seongnam 13120, Korea.
Department of Obstetrics and Gynecology, College of Korean Medicine, Daejeon University, Daejeon 35235, Korea.
Biomolecules. 2021 Jul 18;11(7):1052. doi: 10.3390/biom11071052.
Genistein (4,5,7-trihydroxyisoflavone) is abundant in various dietary vegetables, especially soybeans, and is known to have not only an estrogenic effect but also an antiadipogenic effect. Atorvastatin (dihydroxy monocarboxylic acid) is a statin used to prevent heart disease. Although genistein and atorvastatin have been reported to possess antiadipogenic effects, their combined effects are still unclear. The aim of the current study was to explore whether the combination of genistein and atorvastatin at low concentrations significantly suppresses adipogenesis in a murine preadipocyte cell line (3T3-L1) compared to treatment with genistein or atorvastatin alone. Our results showed that cotreatment with 50 µM genistein and 50 nM atorvastatin significantly suppressed preadipocyte differentiation, whereas when each compound was used alone, there was no inhibitory effect. Additionally, cotreatment with genistein and atorvastatin significantly downregulated adipogenic marker proteins, including mitogen-activated protein kinases (MAPKs), peroxisome proliferator-activated receptor γ (PPARγ), CCAAT/enhancer-binding protein alpha (C/EBPα), glucocorticoid receptor (GR), and CCAAT/enhancer-binding protein β (C/EBPβ). This is the first evidence of the combined antiadipogenic effects of genistein and atorvastatin. Although additional experiments are required, combinational treatment with genistein and atorvastatin may be an alternative treatment for menopause-associated lipid metabolic disorders and obesity.
染料木黄酮（4,5,7-三羟基异黄酮）广泛存在于各种蔬菜中，尤其是大豆，不仅具有雌激素作用，还具有抗脂肪生成作用。阿托伐他汀（二羟基单羧酸）是一种用于预防心脏病的他汀类药物。尽管已报道染料木黄酮和阿托伐他汀具有抗脂肪生成作用，但它们的联合作用仍不清楚。本研究旨在探讨低浓度的染料木黄酮和阿托伐他汀联合使用是否比单独使用染料木黄酮或阿托伐他汀更能显著抑制小鼠前脂肪细胞系（3T3-L1）中的脂肪生成。我们的结果表明，50μM 染料木黄酮和 50nM 阿托伐他汀联合处理可显著抑制前脂肪细胞分化，而单独使用每种化合物时则没有抑制作用。此外，染料木黄酮和阿托伐他汀联合处理可显著下调脂肪生成标志物蛋白，包括丝裂原活化蛋白激酶（MAPKs）、过氧化物酶体增殖物激活受体γ（PPARγ）、CCAAT/增强子结合蛋白α（C/EBPα）、糖皮质激素受体（GR）和 CCAAT/增强子结合蛋白β（C/EBPβ）。这是染料木黄酮和阿托伐他汀联合抗脂肪生成作用的首次证据。尽管需要进一步的实验，但染料木黄酮和阿托伐他汀联合治疗可能是治疗绝经相关脂代谢紊乱和肥胖的一种替代方法。
