基于表面增强拉曼散射的细菌病原体内毒素的高灵敏检测和区分。
Highly Sensitive Detection and Differentiation of Endotoxins Derived from Bacterial Pathogens by Surface-Enhanced Raman Scattering.
机构信息
College of Food Science and Nutritional Engineering, National Engineering Research Center for Fruit & Vegetable Processing, Key Laboratory of Fruit & Vegetable Processing, Ministry of Agriculture, Beijing Key Laboratory for Food Non-Thermal Processing, China Agricultural University, Beijing 100083, China.
Department of Physics and Astronomy, University of Georgia, Athens, GA 30602, USA.
出版信息
Biosensors (Basel). 2021 Jul 11;11(7):234. doi: 10.3390/bios11070234.
Bacterial endotoxins, as major components of Gram-negative bacterial outer membrane leaflets and a well-characterized TLR4-MD-2 ligand, are lipopolysaccharides (LPSs) that are constantly shed from bacteria during growth and infection. For the first time, we report that unique surface-enhanced Raman scattering (SERS) spectra of enteric LPSs from , , , , species . CE3, and . NGR, as well as endotoxin structures, LPSs, lipid A, and KDO2-lipid A can be obtained. The characteristic peaks of the SERS spectra reveal that most of the tested LPS structures are from lipids and saccharides, i.e., the major components of LPSs, and these spectra can be successfully used to differentiate between endotoxins with principal components analysis. In addition, all the LPS samples here are measured at a concentration of 10 nmole/mL, which corresponds to their relevant pathophysiological concentrations in clinical infections. This study demonstrates that LPSs can be used as biomarkers for the highly sensitive detection of bacteria using SERS-based methods.
细菌内毒素作为革兰氏阴性细菌外膜小叶的主要成分和一种特征明确的 TLR4-MD-2 配体，是脂多糖（LPS），在细菌生长和感染过程中不断从细菌中脱落。我们首次报道，来自 、 、 、 、 、CE3 和. NGR 种属的肠道 LPS 具有独特的表面增强拉曼散射（SERS）谱，以及内毒素结构、LPS、脂质 A 和 KDO2-脂质 A。SERS 光谱的特征峰表明，大多数测试的 LPS 结构都来自脂质和糖，即 LPS 的主要成分，这些光谱可以成功地用于主成分分析来区分内毒素。此外，这里所有的 LPS 样品都是在 10nmole/mL 的浓度下测量的，这与它们在临床感染中的相关病理生理浓度相对应。这项研究表明，LPS 可以作为使用基于 SERS 的方法进行细菌高灵敏度检测的生物标志物。
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