Gomes Luciana C, Faria Sara I, Valcarcel Jesus, Vázquez José A, Cerqueira Miguel A, Pastrana Lorenzo, Bourbon Ana I, Mergulhão Filipe J
LEPABE-Laboratory for Process Engineering, Environment, Biotechnology and Energy, Faculty of Engineering, University of Porto, Rua Dr. Roberto Frias, 4200-465 Porto, Portugal.
Grupo de Reciclado y Valorización de Materiales Residuales (REVAL), Instituto de Investigaciones Marinas (IIM-CSIC), C/Eduardo Cabello, 6, CP36208 Vigo, Spain.
Mar Drugs. 2021 Jul 2;19(7):384. doi: 10.3390/md19070384.
The growing requirement for sustainable processes has boosted the development of biodegradable plastic-based materials incorporating bioactive compounds obtained from waste, adding value to these products. Chitosan (Ch) is a biopolymer that can be obtained by deacetylation of chitin (found abundantly in waste from the fishery industry) and has valuable properties such as biocompatibility, biodegradability, antimicrobial activity, and easy film-forming ability. This study aimed to produce and characterize poly(lactic acid) (PLA) surfaces coated with β-chitosan and β-chitooligosaccharides from a pen with different molecular weights for application in the food industry. The PLA films with native and depolymerized Ch were functionalized through plasma oxygen treatment followed by dip-coating, and their physicochemical properties were assessed by Fourier-transform infrared spectroscopy, X-ray diffraction, water contact angle, and scanning electron microscopy. Their antimicrobial properties were assessed against and , where Ch-based surfaces reduced the number of biofilm viable, viable but nonculturable, and culturable cells by up to 73%, 74%, and 87%, respectively, compared to PLA. Biofilm growth inhibition was confirmed by confocal laser scanning microscopy. Results suggest that Ch films of higher molecular weight had higher antibiofilm activity under the food storage conditions mimicked in this work, contributing simultaneously to the reuse of marine waste.
对可持续工艺日益增长的需求推动了基于可生物降解塑料的材料的发展，这些材料包含从废物中获得的生物活性化合物，从而增加了这些产品的价值。壳聚糖（Ch）是一种生物聚合物，可通过几丁质（在渔业废弃物中大量存在）的脱乙酰作用获得，具有生物相容性、生物降解性、抗菌活性和易于成膜等宝贵特性。本研究旨在制备并表征涂覆有不同分子量的β-壳聚糖和β-壳寡糖的聚乳酸（PLA）表面，用于食品工业。通过等离子体氧处理然后浸涂对具有天然和降解Ch的PLA薄膜进行功能化，并通过傅里叶变换红外光谱、X射线衍射、水接触角和扫描电子显微镜评估其物理化学性质。评估了它们对 和 的抗菌性能，与PLA相比，基于Ch的表面分别将生物膜中活细胞、活但不可培养细胞和可培养细胞的数量减少了高达73%、74%和87%。共聚焦激光扫描显微镜证实了生物膜生长的抑制。结果表明，在本研究模拟的食品储存条件下，较高分子量的Ch薄膜具有更高的抗生物膜活性，同时有助于海洋废弃物的再利用。
