Koo Hyun-Na, Choi Jihye, Shin Eungyeong, Kang Wonjin, Cho Sun-Ran, Kim Hyunkyung, Park Bueyong, Kim Gil-Hah
Department of Plant Medicine, College of Agriculture, Life and Environment Sciences, Chungbuk National University, Cheongju 28644, Korea.
Crop Protection Division, National Institute of Agricultural Science, Wanju 55365, Korea.
Insects. 2021 Jul 20;12(7):660. doi: 10.3390/insects12070660.
The two-spotted spider mite Koch is a major agricultural pest worldwide and is known to rapidly develop resistance to pesticides. In the present study, we explored a field strain that was collected in 2000 and 2003 and has been exhibiting resistance to etoxazole and pyridaben over the last 16 years. The resistance ratios of the etoxazole- and pyridaben-resistant strains (ER and PR) to etoxazole or pyridaben were more than 5,000,000- and 4109.6-fold higher than that of the susceptible strain, respectively. All field-collected populations showed resistance to etoxazole and pyridaben. The ER and PR strains showed cross-resistance to several acaricides. Both I1017F and H92R point mutations were detected in 7 out of 8 field groups. Spirodiclofen and spiromesifen resulted in more than 77.5% mortality in the 8 field groups. In addition, the genotype frequency of the I1017F point mutation was 100.0% in the ER strain, and that of the H92R point mutation was 97.0% in the PR strain. All of the field populations were found to have a high frequency of I1017F. These results suggest that the observation of resistance patterns will help in designing a sustainable IPM program for .
二斑叶螨科赫是一种全球主要的农业害虫，已知其对杀虫剂能迅速产生抗性。在本研究中，我们探究了一个于2000年和2003年采集的田间品系，该品系在过去16年中一直表现出对乙螨唑和哒螨灵的抗性。乙螨唑抗性品系（ER）和哒螨灵抗性品系（PR）对乙螨唑或哒螨灵的抗性倍数分别比敏感品系高500万倍和4109.6倍。所有田间采集的种群均对乙螨唑和哒螨灵表现出抗性。ER和PR品系对几种杀螨剂表现出交叉抗性。在8个田间种群中有7个检测到I1017F和H92R点突变。螺螨酯和螺甲螨酯在8个田间种群中导致的死亡率超过77.5%。此外，I1017F点突变在ER品系中的基因型频率为100.0%，H92R点突变在PR品系中的基因型频率为97.0%。所有田间种群均发现I1017F的频率较高。这些结果表明，观察抗性模式将有助于为……设计一个可持续的综合虫害管理计划。
