细胞色素 P450 CYP389C16 有助于朱砂叶螨对氟啶虫胺腈和哒螨灵的交叉耐药性。
The cytochrome P450 CYP389C16 contributes to the cross-resistance between cyflumetofen and pyridaben in Tetranychus cinnabarinus (Boisduval).
机构信息
Key Laboratory of Entomology and Pest Control Engineering, College of Plant Protection, Southwest University, Chongqing, 400716, China.
Academy of Agricultural Sciences, Southwest University, Chongqing, 400716, China.
出版信息
Pest Manag Sci. 2020 Feb;76(2):665-675. doi: 10.1002/ps.5564. Epub 2019 Aug 26.
BACKGROUND
Acaricide resistance is a serious problem in spider mites. Cyflumetofen is a new complex II inhibitor, whereas pyridaben acts at complex I and has been used for decades. Although cross-resistance between cyflumetofen and pyridaben has been observed in Tetranychus cinnabarinus, the specific mechanisms at play have not yet been investigated.
RESULTS
Investigation into the cross-resistance mechanisms identified five P450s, among which CYP389C16 was evaluated as the most likely candidate conferring cross-resistance. Knockdown of CYP389C16 expression via RNA interference diminished the level of cross-resistance in the cyflumetofen-resistant strain. In addition, recombinant CYP389C16 (40 pmol) effectively metabolized 25.0 ± 0.7% of cyflumetofen, 39.7 ± 1.0% of pyridaben, and 69.3 ± 3.3% of AB-1 (active de-esterified metabolite of cyflumetofen) within 2 h. In addition, hydroxylation metabolite of AB-1 was identified by HPLC-MS/MS.
CONCLUSIONS
The study reveals that overexpressed CYP389C16 is involved in the cross-resistance between cyflumetofen and pyridaben in T. cinnabarinus. © 2019 Society of Chemical Industry.
背景
杀螨剂抗性是蜘蛛螨的一个严重问题。氟氯苯菊酯是一种新型的复合物 II 抑制剂，而哒螨灵则作用于复合物 I，并已使用了几十年。尽管在朱砂叶螨中已经观察到氟氯苯菊酯和哒螨灵之间存在交叉抗性，但具体的作用机制尚未得到研究。
结果
对交叉抗性机制的研究确定了五个 P450s，其中 CYP389C16 被评估为最有可能赋予交叉抗性的候选基因。通过 RNA 干扰降低 CYP389C16 的表达水平，降低了氟氯苯菊酯抗性菌株的交叉抗性水平。此外，重组 CYP389C16（40pmol）在 2 小时内有效地代谢了 25.0±0.7%的氟氯苯菊酯、39.7±1.0%的哒螨灵和 69.3±3.3%的 AB-1（氟氯苯菊酯的活性去酯化代谢物）。此外，通过 HPLC-MS/MS 鉴定了 AB-1 的羟化代谢物。
结论
本研究表明，过表达的 CYP389C16 参与了朱砂叶螨中氟氯苯菊酯和哒螨灵之间的交叉抗性。 © 2019 化学工业协会。
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