Centro Interdisciplinario de Ciencias Marinas (IPN-CICIMAR), Av. Instituto Politécnico Nacional s/n, Col. Playa Palo de Santa Rita, La Paz 23096, Mexico.
Centro de Investigaciones Biológicas del Noroeste (CIBNOR), S.C. Instituto Politécnico Nacional 195, Col. Playa Palo Santa Rita, La Paz 23096, Mexico.
Toxins (Basel). 2021 Jul 20;13(7):506. doi: 10.3390/toxins13070506.
Allelopathy between phytoplankton species can promote cellular stress and programmed cell death (PCD). The raphidophyte var. , and the dinoflagellates and have allelopathic effects on ; however, the physiological mechanisms are unknown. We evaluated whether the allelopathic effect promotes cellular stress and activates PCD in . Cultures of were exposed to cell-free media of var. , and . The mortality, superoxide radical (O) production, thiobarbituric acid reactive substances (TBARS) levels, superoxide dismutase (SOD) activity, protein content, and caspase-3 activity were quantified. Mortality (between 57 and 79%) was registered in after exposure to cell-free media of the three species. The maximal O production occurred with var cell-free media. The highest TBARS levels and SOD activity in were recorded with cell-free media from . The highest protein content was recorded with cell-free media from . All cell-free media caused an increase in the activity of caspase-3. These results indicate that the allelopathic effect in promotes cell stress and caspase-3 activation, as a signal for the induction of programmed cell death.
浮游植物种间的化感作用可促进细胞应激和细胞程序性死亡（PCD）。红藻 var. 和腰鞭毛目藻类 和 对 具有化感作用，但生理机制尚不清楚。我们评估了化感作用是否会促进细胞应激并激活 中的 PCD。将 的培养物暴露于 var.、 和 的无细胞培养基中。定量测定死亡率、超氧自由基（O）产生、硫代巴比妥酸反应物质（TBARS）水平、超氧化物歧化酶（SOD）活性、蛋白质含量和半胱天冬酶-3 活性。暴露于三种物种的无细胞培养基后， 中的死亡率（57%至 79%之间）有所登记。O 的最大产生发生在 var 的无细胞培养基中。来自 的无细胞培养基在 中记录到最高的 TBARS 水平和 SOD 活性。来自 的无细胞培养基在 中记录到最高的蛋白质含量。所有无细胞培养基均导致半胱天冬酶-3 活性增加。这些结果表明， 在 中的化感作用可促进细胞应激和半胱天冬酶-3 的激活，作为诱导细胞程序性死亡的信号。
