Centre of Marine Sciences (CCMAR) and Department of Chemistry and Pharmacy, Faculty of Science and Technology, University of Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
GreenCoLab, Green Ocean Association, Universidade do Algarve, Campus de Gambelas, 8005-139 Faro, Portugal.
Toxins (Basel). 2022 Nov 4;14(11):762. doi: 10.3390/toxins14110762.
has been the main species responsible for paralytic shellfish poisoning events along the Portuguese coast (Iberian Peninsula), causing bans on bivalve harvesting that result in huge economic losses. This work presents the characterization of two novel isolates of regarding their growth and toxin profiles. Laboratory growth experiments revealed that, although low growth rates were obtained during cultivation, the cell yields were high compared to those reported in the literature. Evaluation of the toxin profiles, by HPLC-FLD, essentially confirmed the typical composition of toxins of this regional population (Iberian Peninsula), namely, the absence or low representation of the toxins dcNEO, GTX1,4 and NEO and a higher ratio of the toxins C1,2, GTX6 and GTX5. However, the percentage of the identified toxins varied among the strains of this study (under the same isolation, growth, and analysis conditions), and also differed from that of other strains described in the literature. Interestingly, we found a comparatively high abundance of dcSTX in both strains, relative to the other toxins, and an unquantifiable amount of C3,4 toxins. In addition to the geographic relationship between toxin profiles, chemical conversions among toxins may explain some differences encountered in the toxin profiles of strains.
一直是沿葡萄牙海岸（伊比利亚半岛）发生麻痹性贝类毒素中毒事件的主要物种，导致双壳类贝类捕捞禁令，造成巨大的经济损失。本工作对两个新的进行了特征描述，包括它们的生长和毒素特征。实验室生长实验表明，尽管在培养过程中获得的生长速率较低，但与文献报道相比，细胞产率较高。通过 HPLC-FLD 评估毒素特征，基本上证实了该地区种群（伊比利亚半岛）毒素的典型组成，即缺乏或低代表毒素 dcNEO、GTX1,4 和 NEO，以及毒素 C1,2、GTX6 和 GTX5 的比例较高。然而，在这项研究的菌株中（在相同的分离、生长和分析条件下），鉴定出的毒素百分比有所不同，也与文献中描述的其他菌株不同。有趣的是，我们发现两株菌中 dcSTX 的丰度相对较高，与其他毒素相比，C3,4 毒素的含量无法定量。除了毒素特征的地理关系外，毒素之间的化学转化也可能解释了在菌株毒素特征中遇到的一些差异。
