Garbowska Monika, Berthold-Pluta Anna, Stasiak-Różańska Lidia, Pluta Antoni, Forsythe Stephen, Stefańska Ilona
Department of Technology and Food Assessment, Division of Milk Technology, Institute of Food Sciences, Warsaw University of Life Sciences-SGGW, 02-787 Warsaw, Poland.
FoodMicrobe.com, Keyworth, Nottinghamshire NG12 5GY, UK.
Foods. 2023 Oct 23;12(20):3873. doi: 10.3390/foods12203873.
The present study aimed to determine the genotyping diversity and hemolytic properties of 24 strains of spp. (15 , 6 , 2 , and 1 ) isolated from commercial ready-to-eat leaf vegetables, sprouts, nuts, and dried fruits. The multilocus sequence typing (MLST) method was used to determine the sequence types (ST) and clonal complexes (CC) of these strains. The study demonstrated the high genotypic diversity of the genus bacteria isolated from plant-based foods. Five novel sequence types (804, 805, 806, 807, and 808) and the presence of novel alleles in the , , , and loci were detected. In total, 16 of the 24 strains were assigned to the sequence types ST99, ST258, ST17, ST648, ST21, ST494, and ST98. One strain (s12) isolated from alfalfa sprouts was assigned to the clonal complex CC4, which encompasses strains often associated with severe infections leading to meningitis in infants. In addition, 87.5% and 16.7% of the spp. strains showed β-hemolysis of equine and sheep red blood cells, respectively. The presence of the pathogenic species , , and in ready-to-eat plant-derived food products shows they are potential sources of infection, especially to those with compromised immunity, which substantiates their further multi-faceted characterization. The significance of this study may prove useful not only in epidemiological investigations, but also in assessing the risk of infections caused by the presence of .
本研究旨在确定从商业即食叶菜、豆芽、坚果和干果中分离出的24株[细菌名称]（15株[具体菌种1]、6株[具体菌种2]、2株[具体菌种3]和1株[具体菌种4]）的基因分型多样性和溶血特性。采用多位点序列分型（MLST）方法确定这些菌株的序列类型（ST）和克隆复合体（CC）。该研究表明从植物性食品中分离出的[细菌属名]细菌具有高度的基因型多样性。检测到5种新的序列类型（804、805、806、807和808）以及在[具体基因位点1]、[具体基因位点2]、[具体基因位点3]和[具体基因位点4]中存在新的等位基因。24株菌株中共有16株被归类为序列类型ST99、ST258、ST17、ST648、ST21、ST494和ST98。从苜蓿芽中分离出的1株[细菌名称]菌株（s12）被归类为克隆复合体CC4，该复合体包含通常与导致婴儿脑膜炎的严重感染相关的菌株。此外，87.5%和16.7%的[细菌名称]菌株分别对马和绵羊红细胞表现出β溶血。即食植物源性食品中存在致病性菌种[具体菌种1]、[具体菌种2]和[具体菌种3]表明它们是潜在的感染源，尤其是对免疫力低下的人，这证实了对它们进行进一步多方面特征分析的必要性。这项研究的意义可能不仅在流行病学调查中有用，而且在评估由[细菌名称]存在引起的感染风险方面也有用。
