Analytical Chemistry, Applied Chemometrics and Molecular Modelling, Vrije Universiteit Brussel (VUB), Brussels, Belgium.
Medicinal and Aromatic Plants Research Department, Horticulture Institute, Agricultural Research Center, Dokki, Giza, Egypt.
Planta Med. 2021 Oct;87(12-13):1089-1100. doi: 10.1055/a-1554-2733. Epub 2021 Aug 6.
is a common green leafy vegetable that has numerous uses in traditional medicine. This study focused on the determination of the cytotoxic, antiradical, and antidiabetic properties of various fractions of the methanolic extract, as well as on the tentative identification of some bioactive compounds in the same fractions. The cytotoxicity was determined by the brine shrimp lethal test. The antioxidant activities of the fractions were investigated through 3 assays. The antidiabetic activity () was measured by -glucosidase and -amylase inhibition assays. Phytochemical qualitative analyses demonstrated the presence of alkaloids, terpenoids, phenols, and flavonoids in the ethyl acetate-methanol and methanol fractions. The total phenolic and total flavonoid contents were found to be highest in the ethyl acetate-MeOH fractions. The evaluation of the cytotoxicity showed that the hexane-dichloromethane fraction is the most toxic, while the others are moderately toxic. The antioxidant activity assays showed that the ethyl acetate-MeOH fractions are the most potent, while the -glucosidase and -amylase assays revealed that the hexane-dichloromethane fraction might contain a potent antidiabetic agent. Some bioactive substances in the MeOH fractions, such as salicylic acid glucoside, 1-O-sinapoyl--D-glucose derivative, and dihydroferulic acid derivative, were tentatively identified. To the best of our knowledge, this is the first report to detect and identify these compounds in this species. Based on the results of this study, it may be concluded that is a potent antioxidant agent and could be a good candidate as a natural antioxidant in food and therapeutics.
是一种常见的绿叶蔬菜，在传统医学中有多种用途。本研究专注于测定甲醇提取物的各种馏分的细胞毒性、抗自由基和抗糖尿病特性，并暂定鉴定相同馏分中的一些生物活性化合物。细胞毒性通过盐水虾致死试验确定。通过 3 种测定方法研究了馏分的抗氧化活性。通过 -葡萄糖苷酶和 -淀粉酶抑制试验测定抗糖尿病活性。植物化学定性分析表明，乙酸乙酯-甲醇和甲醇馏分中存在生物碱、萜类、酚类和类黄酮。发现乙酸乙酯-MeOH 馏分中的总酚和总类黄酮含量最高。细胞毒性评价表明，正己烷-二氯甲烷馏分毒性最大，而其他馏分毒性中等。抗氧化活性测定表明，乙酸乙酯-MeOH 馏分最有效，而 -葡萄糖苷酶和 -淀粉酶测定表明正己烷-二氯甲烷馏分可能含有有效的抗糖尿病剂。甲醇馏分中的一些生物活性物质，如水杨酸葡萄糖苷、1-O-丁香酰基--D-葡萄糖衍生物和二氢阿魏酸衍生物，被暂定鉴定。据我们所知，这是首次在该物种中检测和鉴定这些化合物。基于本研究的结果，可以得出结论，是一种有效的抗氧化剂，并且可以作为食品和治疗中的天然抗氧化剂的良好候选物。
