Lee Shin Ja, Kim Hyun Sang, Eom Jun Sik, Choi You Young, Jo Seong Uk, Chu Gyo Moon, Lee Yookyung, Seo Jakyeom, Kim Kyoung Hoon, Lee Sung Sill
Institute of Agriculture and Life Science & University-Centered Labs, Gyeongsang National University, Jinju-si 52828, Gyeongsangnam-do, Korea.
Division of Applied Life Science (BK21), Gyeongsang National University, Jinju-si 52828, Gyeongsangnam-do, Korea.
Animals (Basel). 2021 Jul 5;11(7):2008. doi: 10.3390/ani11072008.
We evaluated whether olive leaves (OLs) are effective as feed additives and supplements for ruminants and the potential methane reduction effects during in vitro fermentation. Two Hanwoo cows (460 ± 20 kg) equipped with cannula were fed Timothy hay and corn-based feed 3% of the body weight at a ratio of 6:4 (8:30 a.m. and 5:00 p.m.). Ruminal fluid from the cows was collected and mixed before morning feeding. In vitro batch fermentation was monitored after 12 and 24 h of incubation at 39 °C, and OLs were used as supplements to achieve the concentration of 5% in the basal diet. At 12 h of fermentation, methane production decreased in the 5% OLs group compared to that in the control group, but not at 24 h. The proportion of cellulose-degrading bacteria, , and , tended to increase in the 5% OLs group at 12 h. The amount of ammonia produced was the same as the polymerase chain reaction result for . At 12 h, the proportion of was significantly higher in the 5% OLs group. OLs may be used incorporated with protein byproducts or other methane-reducing agents in animal feed.
我们评估了橄榄叶（OLs）作为反刍动物饲料添加剂和补充剂的有效性以及体外发酵过程中潜在的甲烷减排效果。选用两头装有瘤胃瘘管的韩牛（体重460±20千克），以6:4的比例（上午8:30和下午5:00）饲喂占体重3%的梯牧草和玉米基饲料。在早晨饲喂前收集并混合来自这些牛的瘤胃液。在39℃孵育12小时和24小时后监测体外批次发酵情况，使用OLs作为补充剂以使基础日粮中其浓度达到5%。在发酵12小时时，与对照组相比，5% OLs组的甲烷产量下降，但在24小时时未下降。在12小时时，5% OLs组中纤维素降解菌、和的比例有增加趋势。产生的氨量与的聚合酶链反应结果相同。在12小时时，5% OLs组中的比例显著更高。橄榄叶可与动物饲料中的蛋白质副产品或其他甲烷减排剂混合使用。
