Jo Seong Uk, Lee Shin Ja, Kim Hyun Sang, Eom Jun Sik, Choi Youyoung, Lee Yookyung, Lee Sung Sill
Division of Applied Life Science (BK21), Gyeongsang National University, Jinju 52828, Korea.
Institute of Agriculture & Life Science (IALS), Gyeongsang National University, Jinju 52828, Korea.
Animals (Basel). 2022 Aug 29;12(17):2222. doi: 10.3390/ani12172222.
Ruminants produce large amounts of methane as part of their normal digestive processes. Recently, feed additives were shown to inhibit the microorganisms that produce methane in the rumen, consequently reducing methane emissions. The objective of this study was to evaluate the dose-response effect of var. (PHN) and supplementation on in vitro rumen fermentation, methane, and carbon dioxide production, and the microbial population. An in vitro batch culture system was used, incubated without bamboo leaves (control) or with bamboo leaves (0.3, 0.6, and 0.9 g/L). After 48 h, total gas, methane, and carbon dioxide production decreased linearly with an increasing dose of bamboo leaves supplementation. The total volatile fatty acid, acetate, and acetate-to-propionate ratio were affected quadratically with increasing doses of bamboo leaves supplementation. In addition, propionate decreased linearly. Butyrate was increased linearly with increasing doses of PHN supplementation. The absolute values of total bacteria and methanogenic archaea decreased linearly and quadratically with an increasing dose of PHN treatment after 48 h. These results show that bamboo leaves supplementation can reduce methane production by directly affecting methanogenic archaea, depressing the metabolism of methanogenic microbes, or transforming the composition of the methanogenic community. These results need to be validated using in vivo feeding trials before implementation.
反刍动物在其正常消化过程中会产生大量甲烷。最近，有研究表明饲料添加剂可抑制瘤胃中产生甲烷的微生物，从而减少甲烷排放。本研究的目的是评估变种（PHN）和添加物对体外瘤胃发酵、甲烷和二氧化碳产生以及微生物种群的剂量反应效应。采用体外批次培养系统，在不添加竹叶（对照）或添加竹叶（0.3、0.6和0.9 g/L）的条件下进行培养。48小时后，随着竹叶添加剂量的增加，总气体、甲烷和二氧化碳的产生呈线性下降。随着竹叶添加剂量的增加，总挥发性脂肪酸、乙酸和乙酸与丙酸的比例受到二次影响。此外，丙酸呈线性下降。随着PHN添加剂量的增加，丁酸呈线性增加。48小时后，随着PHN处理剂量的增加，总细菌和产甲烷古菌的绝对值呈线性和二次下降。这些结果表明，添加竹叶可通过直接影响产甲烷古菌、抑制产甲烷微生物的代谢或改变产甲烷群落的组成来减少甲烷产生。在实施之前，这些结果需要通过体内饲养试验进行验证。
