Panja Kamonporn, Buranapraditkun Supranee, Roytrakul Sittiruk, Kovitvadhi Attawit, Lertwatcharasarakul Preeda, Nakagawa Takayuki, Limmanont Chunsumon, Jaroensong Tassanee
Department of Companion Animal Clinical Sciences, Faculty of Veterinary Medicine, Kasetsart University, Bangkhen Campus, Bangkok 10900, Thailand.
Faculty of Veterinary Medicine, Rajamangala University of Technology Tawan-ok, Bangpra, Chonburi 20110, Thailand.
Animals (Basel). 2021 Jul 16;11(7):2119. doi: 10.3390/ani11072119.
The most common neoplasms in intact female dogs are CMGTs. BmKn-2, an antimicrobial peptide, is derived from scorpion venom and has published anticancer effects in oral and colon human cancer cell lines. Thus, it is highly likely that BmKn-2 could inhibit CMGT cell lines which has not been previously reported. This study investigated the proliferation and apoptotic properties of BmKn-2 via Bax and Bcl-2 relative gene expression in two CMGT cell lines, metastatic (CHMp-5b) and non-metastatic (CHMp-13a). The results showed that BmKn-2 inhibited proliferation and induced apoptosis in the CMGT cell lines. The cell morphology clearly changed and increased apoptosis in a dose dependent of manner. The half maximum inhibitory concentration (IC) was 30 µg/mL for CHMp-5b cell line and 54 µg/mL for CHMp-13a cell line. The induction of apoptosis was mediated through Bcl-2 and Bax expression after BmKn-2 treatment. In conclusion, BmKn-2 inhibited proliferation and induced apoptosis in both CHMp-5b and CHMp-13a cell lines via down-regulation of Bcl-2 and up-regulation of Bax relative mRNA expression. Therefore, BmKn-2 could be feasible as candidate treatment for CMGTs.
未绝育母犬中最常见的肿瘤是乳腺肿瘤（CMGTs）。抗菌肽BmKn-2源自蝎毒，已发表的研究表明其对人口腔和结肠癌细胞系具有抗癌作用。因此，BmKn-2极有可能抑制CMGT细胞系，而此前尚无相关报道。本研究通过检测Bax和Bcl-2相关基因表达，研究了BmKn-2对两种CMGT细胞系（转移性CHMp-5b和非转移性CHMp-13a）增殖和凋亡特性的影响。结果显示，BmKn-2抑制了CMGT细胞系的增殖并诱导其凋亡。细胞形态明显改变，凋亡呈剂量依赖性增加。CHMp-5b细胞系的半数最大抑制浓度（IC）为30 µg/mL，CHMp-13a细胞系为54 µg/mL。BmKn-2处理后，凋亡的诱导是通过Bcl-2和Bax的表达介导的。总之，BmKn-2通过下调Bcl-2和上调Bax相对mRNA表达，抑制了CHMp-5b和CHMp-13a细胞系的增殖并诱导其凋亡。因此，BmKn-2有望成为治疗CMGTs的候选药物。
