Flórez M M, Fêo H B, da Silva G N, Yamatogi R S, Aguiar A J, Araújo J P, Rocha N S
Department of Veterinary Clinics, Faculty of Veterinary Medicine, São Paulo State University-UNESP, Botucatu, Brazil.
Veterinary Pathology Research Group, Faculty of Agricultural Sciences, Universidad de Caldas, Manizales, Colombia.
Vet Comp Oncol. 2017 Sep;15(3):793-807. doi: 10.1111/vco.12220. Epub 2016 Feb 16.
Transmissible venereal tumour (TVT) generally presents different degrees of aggressiveness, which makes them unresponsive to conventional treatment protocols. This implies a progressive alteration of their biological profile. This study aimed to evaluate the cytotoxicity, cell survival, apoptosis and cell cycle alterations in TVT cell cultures subjected to treatment with vincristine. Similarly, it assessed possible implications of MDR-1, TP53, BCL-2, and BAX gene expressions in eight TVT primary cultures for both resistance to chemotherapy and biological behaviour. When comparing TVT cells receiving vincristine to those untreated, a statistical difference related to increased cytotoxicity and decreased survival rates, and alterations in G1 and S cell cycle phases were found but without detectable differences in apoptosis. Increased MDR-1 gene expression was observed after treatment. The groups did not differ statistically in relation to the TP53, BAX and BCL-2 genes. Although preliminary, the findings suggest that such augmented expression is related to tumour malignancy and chemotherapy resistance.
传染性性病肿瘤（TVT）通常表现出不同程度的侵袭性，这使得它们对传统治疗方案无反应。这意味着其生物学特性会逐渐改变。本研究旨在评估长春新碱处理的TVT细胞培养物中的细胞毒性、细胞存活、凋亡及细胞周期改变。同样，它评估了MDR-1、TP53、BCL-2和BAX基因表达在8种TVT原代培养物中对化疗耐药性和生物学行为的可能影响。将接受长春新碱处理的TVT细胞与未处理的细胞进行比较时，发现与细胞毒性增加、存活率降低以及G1和S细胞周期阶段改变相关的统计学差异，但凋亡方面未检测到差异。处理后观察到MDR-1基因表达增加。两组在TP53、BAX和BCL-2基因方面无统计学差异。尽管是初步研究，但这些发现表明这种增强的表达与肿瘤恶性程度和化疗耐药性有关。
