Wu Yuping, Wang Jia, Zhou Yong, Qi Yonghua, Ma Licai, Wang Xuannian, Tao Xiaoqi
College of Life Science and Basic Medicine, Xinxiang University, Xinxiang 453003, China.
College of Food Science, Southwest University, Chongqing 400715, China.
Foods. 2021 Jul 20;10(7):1668. doi: 10.3390/foods10071668.
Due to their facile synthesis and friendly functionalization, gold nanoparticles (AuNPs) have been applied in all kinds of biosensors. More importantly, these biosensors, with the combination of AuNPs and immunoassay, are expected to be used for the detection of different compounds with low concentrations in complex samples. In this study, a AuNPs-labeled antibody immunoprobe was prepared and combined with a fluorescence-quenching principle and a background fluorescence-quenching immunochromatographic assay (bFQICA), achieving rapid on-site detection. By using a portable fluorescence immunoquantitative analyzer and a QR code with a built-in standard curve, the rapid quantitative determination for nitrofurazone metabolite of semicarbazide (SEM) in animal-derived foods was realized. The limits of detection (LODs) for bFQICA in egg, chicken, fish, and shrimp were 0.09, 0.10, 0.12, and 0.15 μg kg for SEM, respectively, with the linear range of 0.08-0.41 μg L, the recoveries ranging from 73.5% to 109.2%, and the coefficient of variation <15%, only taking 13 min for the SEM detection. The analysis of animal-derived foods by bFQICA complied with that of liquid chromatography-tandem mass spectrometry (LC-MS/MS).
由于金纳米粒子（AuNPs）易于合成且功能化友好，已被应用于各种生物传感器中。更重要的是，这些将AuNPs与免疫分析相结合的生物传感器有望用于检测复杂样品中低浓度的不同化合物。在本研究中，制备了一种AuNPs标记的抗体免疫探针，并结合荧光猝灭原理和背景荧光猝灭免疫层析分析（bFQICA），实现了快速现场检测。通过使用便携式荧光免疫定量分析仪和内置标准曲线的二维码，实现了动物源食品中呋喃西林代谢物氨基脲（SEM）的快速定量测定。bFQICA对鸡蛋、鸡肉、鱼肉和虾肉中SEM的检测限分别为0.09、0.10、0.12和0.15μg/kg，线性范围为0.08 - 0.41μg/L，回收率为73.5%至109.2%，变异系数<15%，SEM检测仅需13分钟。bFQICA对动物源食品的分析结果与液相色谱 - 串联质谱法（LC-MS/MS）相符。
