College of Science, China Agricultural University, Beijing 100083, China.
College of Engineering, China Agricultural University, Beijing 100083, China.
Int J Mol Sci. 2021 Jul 23;22(15):7857. doi: 10.3390/ijms22157857.
Kinesin-5 motor consists of two pairs of heads and tail domains, which are situated at the opposite ends of a common stalk. The two pairs of heads can bind to two antiparallel microtubules (MTs) and move on the two MTs independently towards the plus ends, sliding apart the two MTs, which is responsible for chromosome segregation during mitosis. Prior experimental data showed that the tails of kinesin-5 Eg5 can modulate the dynamics of single motors and are critical for multiple motors to generate high steady forces to slide apart two antiparallel MTs. To understand the molecular mechanism of the tails modulating the ability of Eg5 motors, based on our proposed model the dynamics of the single Eg5 with the tails and that without the tails moving on single MTs is studied analytically and compared. Furthermore, the dynamics of antiparallel MT sliding by multiple Eg5 motors with the tails and that without the tails is studied numerically and compared. Both the analytical results for single motors and the numerical results for multiple motors are consistent with the available experimental data.
动力蛋白-5 由两对头和尾部组成，它们位于一个共同的柄的两端。这两对头可以结合到两条相互平行的微管（MT）上，并独立地朝着正极端移动，将两条 MT 拉开，这负责有丝分裂过程中的染色体分离。先前的实验数据表明，动力蛋白-5 Eg5 的尾部可以调节单个马达的动力学，对于多个马达产生高稳态力来拉开两条相互平行的 MT 至关重要。为了理解尾部调节 Eg5 马达能力的分子机制，基于我们提出的模型，分析并比较了带有尾部和不带尾部的单个 Eg5 在单个 MT 上的运动动力学。此外，还对带有尾部和不带尾部的多个 Eg5 马达的相互平行 MT 滑动动力学进行了数值研究和比较。单个马达的分析结果和多个马达的数值结果都与现有实验数据一致。
