Laboratory of Pathogen Host Interactions, Université de Montpellier, CNRS, UMR 5235, 34000 Montpellier, France.
IGMM, Univ Montpellier, CNRS, 34293 Montpellier, France.
Int J Mol Sci. 2021 Jul 28;22(15):8108. doi: 10.3390/ijms22158108.
is a commensal bacterium that causes severe infections in soft tissue and the bloodstream. During infection, manipulates host cell response to facilitate its own replication and dissemination. Here, we show that significantly decreases the level of SUMOylation, an essential post-translational modification, in infected macrophages 24 h post-phagocytosis. The reduced level of SUMOylation correlates with a decrease in the SUMO-conjugating enzyme Ubc9. The over-expression of SUMO proteins in macrophages impaired bacterial intracellular proliferation and the inhibition of SUMOylation with ML-792 increased it. Together, these findings demonstrated for the first time the role of host SUMOylation response toward infection.
是一种共生菌，可导致软组织和血液感染。在感染过程中，操纵宿主细胞反应以促进自身复制和传播。在这里，我们表明，在吞噬后 24 小时，感染的巨噬细胞中 SUMOylation 的水平显著降低，SUMOylation 是一种必要的翻译后修饰。这种低水平的 SUMOylation与 SUMO 连接酶 Ubc9 的减少相关。巨噬细胞中 SUMO 蛋白的过表达会损害细菌的细胞内增殖，而用 ML-792 抑制 SUMOylation 则会增加其增殖。这些发现首次证明了宿主 SUMOylation 反应在 感染中的作用。
