一种在鼠抗Sm单克隆抗体及系统性红斑狼疮患者血清抗体上表达的共同独特型。
A common idiotype expressed on a murine anti-Sm monoclonal antibody and antibodies in SLE sera.
作者信息
Takei M, Dang H, Talal N
机构信息
Department of Medicine, University of Texas Health Science Center, Audie L. Murphy Memorial Veterans Administration Hospital, San Antonio 78284-7874.
出版信息
Clin Exp Immunol. 1987 Dec;70(3):546-54.
A rabbit anti-idiotypic antiserum made against a murine monoclonal anti-Sm autoantibody (Y2) was used in a solid-phase radioimmunoassay to investigate idiotypic cross-reactivity among anti-Sm antibodies present in sera from patients with systemic lupus erythematosus. Sera from 25 of 51 SLE patients (49%) containing anti-Sm antibodies were positive for this Y2 idiotype compared to only one of 22 normal human sera. Nine of 28 SLE patients (32%) whose sera were anti-Sm negative were also positive for the Y2 idiotype in low titre. Binding was not due to rheumatoid factor-like activity but was specific for the Y2 determinant and could be eliminated by absorption with Y2 monoclonal antibodies. The anti-idiotypic antibody blocked the ability of 12 of 25 anti-Sm positive lupus sera to bind Sm. Conversely, Sm antigen inhibited the binding of anti-idiotypic antibody in nine of 12 lupus sera.
用针对鼠抗Sm自身抗体（Y2）制备的兔抗独特型抗血清，通过固相放射免疫测定法研究系统性红斑狼疮患者血清中抗Sm抗体间的独特型交叉反应性。51例含抗Sm抗体的系统性红斑狼疮患者中，25例（49%）的血清对该Y2独特型呈阳性反应，而22例正常人血清中只有1例呈阳性。28例抗Sm阴性的系统性红斑狼疮患者中，9例（32%）血清对Y2独特型也呈低滴度阳性。结合并非由类风湿因子样活性所致，而是对Y2决定簇具有特异性，并且可用Y2单克隆抗体吸收消除。抗独特型抗体阻断了25例抗Sm阳性狼疮血清中12例与Sm结合的能力。相反，Sm抗原抑制了12例狼疮血清中9例抗独特型抗体的结合。
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