Pisetsky D S, Lerner E A, Caster S
Mol Immunol. 1983 Jun;20(6):615-21. doi: 10.1016/0161-5890(83)90006-8.
Y2, a monoclonal anti-Sm antibody of MRL origin, demonstrates an idiotype commonly expressed in autoimmune MRL mice, although not necessarily associated with anti-Sm activity. To identify non-anti-Sm antibodies with this common idiotype, a rabbit anti-Y2 anti-idiotypic antiserum was tested for activity with other MRL hybridoma products (HP) as well as BALB/c myelomas. Idiotypic cross-reactivity was thus demonstrated for Y2 and the product of the BALB/c fusing cell line 45.6TG1.7 (M45), an antibody without known antigen binding activity. This determinant was denoted the Y2-M45 idiotype and its presence in serum and other antibodies tested by an inhibition ELISA. The idiotype was demonstrated on some, but not all, MRL HP's tested and was not confined to antibodies of a unique specificity. The idiotype was also present in sera of some normal as well as autoimmune strains of mice with highest levels in two strains bearing the lpr gene. These results indicate that idiotypes of anti-Sm antibodies are not exclusive to the pathologic setting but may be found commonly expressed in mice with antibodies of a variety of binding activity.
Y2是一种源自MRL的抗Sm单克隆抗体，它表现出一种在自身免疫性MRL小鼠中常见的独特型，尽管不一定与抗Sm活性相关。为了鉴定具有这种常见独特型的非抗Sm抗体，用兔抗Y2抗独特型抗血清检测其与其他MRL杂交瘤产物（HP）以及BALB/c骨髓瘤的活性。由此证明Y2与BALB/c融合细胞系45.6TG1.7（M45）的产物存在独特型交叉反应，M45是一种无已知抗原结合活性的抗体。这个决定簇被命名为Y2-M45独特型，并通过抑制ELISA检测其在血清和其他抗体中的存在情况。在一些但不是所有测试的MRL HP中都证明了这种独特型，并且不限于具有独特特异性的抗体。在一些正常小鼠以及自身免疫性小鼠品系的血清中也存在这种独特型，在携带lpr基因的两个品系中水平最高。这些结果表明，抗Sm抗体的独特型并非病理性环境所特有，而是可能在具有各种结合活性抗体的小鼠中普遍表达。
