Bektas Ezgi Irem, Gurel Pekozer Gorke, Kök Fatma Neşe, Torun Kose Gamze
Department of Genetics and Bioengineering, Faculty of Engineering, Yeditepe University, Istanbul 34755, Turkey.
Department of Biomedical Engineering, Faculty of Electrical and Electronics Engineering, Yildiz Technical University, Istanbul 34220, Turkey.
Carbohydr Polym. 2021 Nov 1;271:118407. doi: 10.1016/j.carbpol.2021.118407. Epub 2021 Jul 8.
In this study, three natural biomaterials, Locust bean gum (LBG), Xanthan gum (XG), and Mastic gum (MG), were combined to form cryogel scaffolds. Thermal and chemical characterizations revealed the successful blend formation from LBG-XG (LX) and LBG-XG-MG (LXM) polymers. All blends resulted in macro-porous scaffolds with interconnected pore structures under the size of 400 μm. The swollen cryogels had similar mechanical properties compared with other polysaccharide-based cryogels. The mean tensile and compressive modulus values of the wet cryogels were in the range of 3.5-11.6 kPa and 82-398 kPa, respectively. The sustained release of the small molecule Kartogenin from varying concentrations and ratios of cryogels was in between 32 and 66% through 21 days of incubation. Physical, mechanical, and chemical properties make LX and LXM polysaccharide-based cryogels promising candidates for cartilage and other soft tissue engineering, and drug delivery applications.
在本研究中，三种天然生物材料，刺槐豆胶（LBG）、黄原胶（XG）和乳香胶（MG）被组合形成冷冻凝胶支架。热学和化学表征揭示了由LBG-XG（LX）和LBG-XG-MG（LXM）聚合物成功形成共混物。所有共混物都形成了具有相互连通的孔隙结构且孔径小于400μm的大孔支架。与其他基于多糖的冷冻凝胶相比，溶胀后的冷冻凝胶具有相似的力学性能。湿冷冻凝胶的平均拉伸模量和压缩模量值分别在3.5 - 11.6kPa和82 - 398kPa范围内。通过21天的孵育，小分子软骨素从不同浓度和比例的冷冻凝胶中的持续释放率在32%至66%之间。物理、力学和化学性质使基于LX和LXM多糖的冷冻凝胶成为软骨和其他软组织工程以及药物递送应用的有前景的候选材料。
