Department of Pulmonary Medicine, Affiliated Hospital of Jiangsu University, Zhenjiang, Jiangsu, China.
Department of Respiratory Disease, Yancheng Third People's Hospital, Yancheng, Jiangsu, China.
Cancer Biomark. 2022;33(1):71-82. doi: 10.3233/CBM-210090.
Differential diagnosis between malignant pleural effusion (MPE) and benign pleural effusion (BPE) remains a clinical challenge.
The aim of the study is to assess the efficacy of the serum and pleural fluid (PF) miRNA panels in distinguishing MPE from BPE.
Fourteen candidate miRNAs which were shown aberrant expression in lung cancer based on previous studies were tested by quantitative real-time PCR (qRT-PCR) in 20 MPE patients and 20 BPE patients. Significantly aberrantly expressed miRNAs were further assessed by qRT-PCR in all patients enrolled in this study. A receiver operating characteristic (ROC) curve was constructed, and the area under the ROC curve (AUC) was calculated to evaluated the diagnostic performance of the miRNAs.
miR-21, miR-29c and miR-182 were found to be significantly aberrantly expressed in the serum and PF of MPE patients. The AUCs for the combination of miR-21, miR-29c and miR-182 in serum and PF were 0.832 and 0.89 respectively in distinguishing MPE from infection-associated PE including tuberculous pleurisy and parapneumonia PE, and 0.866 and 0.919 respectively for differentiating MPE from heart failure-associated PE, which were superior to AUC of each individual miRNAs.
miR-21, miR-29c and miR-182 in serum and PF could be useful biomarkers for diagnosis of MPE.
恶性胸腔积液（MPE）与良性胸腔积液（BPE）的鉴别诊断仍然是临床面临的挑战。
本研究旨在评估血清和胸腔积液（PF）miRNA 谱在鉴别 MPE 与 BPE 中的作用。
根据以往的研究，通过定量实时 PCR（qRT-PCR）在 20 名 MPE 患者和 20 名 BPE 患者中检测了 14 种候选 miRNA，这些 miRNA 在肺癌中表现出异常表达。在本研究纳入的所有患者中，进一步通过 qRT-PCR 评估显著异常表达的 miRNA。构建受试者工作特征（ROC）曲线，计算 ROC 曲线下面积（AUC），以评估 miRNA 的诊断性能。
miR-21、miR-29c 和 miR-182 在 MPE 患者的血清和 PF 中均发现存在显著异常表达。miR-21、miR-29c 和 miR-182 在血清和 PF 联合鉴别结核性胸膜炎和肺炎旁胸腔积液等感染性胸腔积液与 MPE 时的 AUC 分别为 0.832 和 0.89，与心力衰竭相关胸腔积液与 MPE 时的 AUC 分别为 0.866 和 0.919，均优于单个 miRNA 的 AUC。
血清和 PF 中的 miR-21、miR-29c 和 miR-182 可作为 MPE 诊断的有用生物标志物。
