雄性可育与雄性不育苜蓿之间的比较转录组（）
Comparative transcriptome between male fertile and male sterile alfalfa ().
作者信息
Wang Jia, Tang Fang, Gao Cuiping, Gao Xia, Xu Bo, Shi Fengling
机构信息
Key Laboratory of Grassland Resources, College of Grassland, Resources and Environment, Inner Mongolia Agricultural University, No.29 Erdos Street, Saihan District, Hohhot, 010011 Inner Mongolia China.
Department of Pharmacy, Baotou Medical College, Baotou, 014040 Inner Mongolia China.
出版信息
Physiol Mol Biol Plants. 2021 Jul;27(7):1487-1498. doi: 10.1007/s12298-021-01026-x. Epub 2021 Jun 29.
UNLABELLED
Male sterility is an important factor in improving crop quality and yield through heterosis breeding. In this study, we analyzed the transcriptomes of male fertile (MF) and male sterile (MS) alfalfa flower buds using the Illumina HiSeq™ 4000 platform. A total of 54.05 million clean reads were generated and assembled into 65,777 unigenes with an average length of 874 bp. The differentially expressed genes (DEGs) between the MF and MS flowers at three stages of pollen development were identified, and there were 3832, 5678 and 5925 DEGs respectively in stages 1, 2 and 3. GO and KEGG functional enrichment analysis revealed 12, 12, 6 and 12 key branch-point genes involved in circadian rhythm, transcription factors, pollen development and flavonoid biosynthesis. Our findings provide novel insights into the mechanism of male sterility in alfalfa.
SUPPLEMENTARY INFORMATION
The online version contains supplementary material available at 10.1007/s12298-021-01026-x.
未标注
雄性不育是通过杂种优势育种提高作物品质和产量的重要因素。在本研究中，我们使用Illumina HiSeq™ 4000平台分析了雄性可育（MF）和雄性不育（MS）苜蓿花芽的转录组。共产生了5405万个clean reads，并组装成65777个单基因，平均长度为874 bp。鉴定了花粉发育三个阶段MF和MS花之间的差异表达基因（DEG），在第1、2和3阶段分别有3832、5678和5925个DEG。GO和KEGG功能富集分析揭示了参与昼夜节律、转录因子、花粉发育和类黄酮生物合成的12、12、6和12个关键分支点基因。我们的研究结果为苜蓿雄性不育机制提供了新的见解。
补充信息
在线版本包含可在10.1007/s12298-021-01026-x获取的补充材料。
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