Tang Nan, Liu Wei, Zhang Wuhua, Tang Daocheng
Plateau Flower Research Centre, Department of Agriculture and Husbandry, Qinghai University, Xining, 810016 People's Republic of China.
State Key Laboratory of Plateau Ecology and Agriculture, Qinghai University, Xining, 810016 People's Republic of China.
Physiol Mol Biol Plants. 2020 Oct;26(10):2061-2074. doi: 10.1007/s12298-020-00886-z. Epub 2020 Sep 25.
Male sterile and male fertile two-type lines are important in heterosis utilization and breeding in , but the genes and pathways involved in male sterility are poorly understood. To explore these topics, transcriptome data (by RNA-seq) and proteome data (by iTRAQ) were gathered from flower buds of the male sterile line 'MS2-2' and male fertile line 'MF2-2' and integrated for a better understanding of the underlying molecular mechanisms of male sterility in . The RNA-seq procedure generated 285,139,740 clean reads and 63359 unigenes and 6640 differentially expressed genes (DEGs) were identified, of which 4136 were downregulated and 2504 were upregulated in 'MS2-2'. DEGs related to flower development, pollen development, pollen wall assembly, endogenous hormones and transcription factors were identified. The iTRAQ analysis identified 3950 proteins in total; 789 were differentially expressed proteins (381 upregulated, 408 downregulated), which were mainly annotated to the Ribosome, Carbon metabolism and Biosynthesis of amino acids pathways. An association analysis revealed strong correlation (r Pearson = 0.6019) between the transcriptomic and proteomic data, and 256 and 34 proteins showed the same and opposite expression patterns with regard to their transcripts, respectively. Pathways such as photosynthesis, fatty acid biosynthesis and phenylpropanoid biosynthesis which influence tapetum and pollen development in male sterile plants, were significantly enriched at the transcript and protein levels. Most genes involved in these pathways were downregulated in 'MS2-2'. The low expression of these genes or functional loss of proteins could be associated with flower development, pollen development and related to changes in fertility in . This study provided transcriptomic and proteomic information for that could illuminate the mechanism of male sterility.
雄性不育系和雄性可育两系在杂种优势利用和育种中具有重要意义，但雄性不育所涉及的基因和途径尚不清楚。为了探究这些问题，从雄性不育系‘MS2-2’和雄性可育系‘MF2-2’的花芽中收集了转录组数据（通过RNA测序）和蛋白质组数据（通过iTRAQ），并进行整合，以更好地理解雄性不育的潜在分子机制。RNA测序程序产生了285,139,740条clean reads和63359个单基因，鉴定出6640个差异表达基因（DEGs），其中在‘MS2-2’中4136个下调，2504个上调。鉴定出与花发育、花粉发育、花粉壁组装、内源激素和转录因子相关的DEGs。iTRAQ分析共鉴定出3950种蛋白质；789种为差异表达蛋白质（381种上调，408种下调），主要注释到核糖体、碳代谢和氨基酸生物合成途径。关联分析显示转录组和蛋白质组数据之间存在强相关性（皮尔逊r = 0.6019），分别有256和34种蛋白质在转录本方面表现出相同和相反的表达模式。影响雄性不育植物绒毡层和花粉发育的光合作用、脂肪酸生物合成和苯丙烷生物合成等途径在转录和蛋白质水平上显著富集。参与这些途径的大多数基因在‘MS2-2’中下调。这些基因的低表达或蛋白质功能丧失可能与花发育、花粉发育以及育性变化有关。本研究为阐明雄性不育机制提供了转录组和蛋白质组信息。
