Pneumococcal antigens and endotoxin in effusions from patients with secretory otitis media.

Counterimmunoelectrophoresis revealed pneumococcus capsular polysaccharide antigen in 16% of 108 effusions from chronic secretory otitis media (SOM). Pneumococci were cultured from the effusion in only 1%. In an additional series of 23 SOM ears, only 4.3% were antigen-positive. Tests by means of pneumococcal coagglutination and C-polysaccharide detection gave identical results. Endotoxin was detected in 76% of the specimens. Control tests with aggregated NHS, IgG and IgA diluted 1: 10 were invariably positive. It appears that endotoxin positivity in SOM fluids results from the presence of activated immunoglobulins, most probably dimeric IgA, in the secretion, and so allows no conclusions as to the bacterial etiology of SOM.