KLF4和KLF5在胃肠道肿瘤中的部分作用

The Partial Role of KLF4 and KLF5 in Gastrointestinal Tumors.

作者信息

Li Jun-Chen, Chen Qiu-Han, Jian Rui, Zhou Jiang-Rong, Xu Yun, Lu Fang, Li Jun-Qiao, Zhang Hao

机构信息

Department of Gastroenterology, The Second Affiliated Hospital of Chongqing Medical University, Chongqing 400010, China.

Blood Purification Center, University-Town Hospital of Chongqing Medical University, Chongqing 400000, China.

出版信息

Gastroenterol Res Pract. 2021 Jul 27;2021:2425356. doi: 10.1155/2021/2425356. eCollection 2021.

BACKGROUND

KLF4 and KLF5 are members of the KLF transcription factor family, which play an important role in many gastrointestinal tumors. To gain a deeper insight into its function and role, bioinformatics was used to analyze the function and role of KLF4 and KLF5 in gastrointestinal tumors.

METHODS

Data were collected from several online databases. Gene Expression Profiling Interactive Analysis (GEPIA), UALCAN database analysis, Kaplan-Meier Plotter analysis, LOGpc system, the Pathology Atlas, and the STRING website were used to analyze the data. We download relevant data from TCGA and then perform GO enrichment and KEGG enrichment analysis. The effects of KLF5 on gastric cancer cell proliferation were measured by CCK-8 assay. The effect of KLF5 on the expression of CyclinD1 and MMP9 was detected by Western blot.

RESULTS

KLF4 and KLF5 were differentially expressed in normal and tumor tissues of the gastrointestinal tract, and their differential expression is related to several genes or pathways. KEGG analysis showed that KLF5 was coexpressed with endocytosis-related genes. KLF5 promotes the proliferation of gastric cancer cells and the expression of metastasis-related molecules.

CONCLUSION

KLF4 and KLF5 are of great significance for developing gastrointestinal tumors and can be used as therapeutic targets.

背景

KLF4和KLF5是KLF转录因子家族的成员，在许多胃肠道肿瘤中发挥重要作用。为了更深入地了解其功能和作用，采用生物信息学方法分析KLF4和KLF5在胃肠道肿瘤中的功能和作用。

方法

从多个在线数据库收集数据。使用基因表达谱交互式分析（GEPIA）、UALCAN数据库分析、Kaplan-Meier Plotter分析、LOGpc系统、病理学图谱和STRING网站对数据进行分析。我们从TCGA下载相关数据，然后进行GO富集和KEGG富集分析。通过CCK-8检测法检测KLF5对胃癌细胞增殖的影响。通过蛋白质免疫印迹法检测KLF5对细胞周期蛋白D1（CyclinD1）和基质金属蛋白酶9（MMP9）表达的影响。