Department of Oral Biology, Faculty of Dentistry Universitas Indonesia, Jakarta, DKI, 10430, Indonesia.
Oral Biology Department, Faculty of Dentistry Universitas Syah Kuala, Banda Aceh, Nagroe Aceh, 23111, Indonesia.
F1000Res. 2021 May 11;10:372. doi: 10.12688/f1000research.52266.3. eCollection 2021.
This study explores the influence of precoating aptamer (Ca-apt1) on viability while the fungus was growing in the presence of exposing condensed cigarette smoke (CSC), prepared from clove (CCSC) and non-clove (NCSC) cigarettes, for 48 h. Using qPCR, we found that mRNA expression of adhesion-associated genes ( ) was impaired by precoating yeast cells with the aptamer. Conversely, the gene transcription was upregulated when aptamer-uncoated yeast was pre-treated with either CSC. In addition, by analysing the result of MTT ([3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H-tetrazolium bromide] assay, we found that the presence of added CCSC or NCSC in growth medium for 48 h was significantly enhanced biofilm development. However, the presence of precoated aptamer was significantly impaired biofilm development accelerated by the NCSC. The inhibitory effect of the Ca-apt1 was not dependent on the precoated aptamer (1ng/μL and 10 ng/μL). Interestingly, we noted that the enhancer effect of treated CCSC was no longer effective when the yeast had been precoated with 10 ng/μL aptamer tested. Additionally, light microscopy analysis revealed that precoating aptamer alleviates morphological changes of (from yeast to hypha formation) that are enhanced by adding CCSC or NCSC in the growth medium. In conclusion, these results suggest that administration on Ca-ap1 exhibits a significant protective effect on CSC-induced biofilm formation by .
本研究探讨了在暴露于丁香（CCSC）和非丁香（NCSC）香烟制备的浓缩香烟烟雾（CSC）存在的情况下，预先包被适配体（Ca-apt1）对真菌生长活力的影响，暴露时间为 48 小时。通过 qPCR，我们发现用适配体预先包被酵母细胞会损害粘附相关基因（）的 mRNA 表达。相反，当未包被的酵母预先用 CSC 处理时，基因转录被上调。此外，通过分析 MTT（[3-(4,5-二甲基-2-噻唑基)-2,5-二苯基-2H-四唑溴盐]测定）结果，我们发现添加的 CCSC 或 NCSC 在生长培养基中存在 48 小时会显著增强生物膜的发展。然而，预先包被的适配体的存在显著抑制了由 NCSC 加速的生物膜形成。Ca-apt1 的抑制作用不依赖于预先包被的适配体（1ng/μL 和 10 ng/μL）。有趣的是，我们注意到，当用 10 ng/μL 适配体预先包被酵母时，用 CCSC 处理的增强作用不再有效。此外，荧光显微镜分析显示，预先包被适配体减轻了由添加 CCSC 或 NCSC 到生长培养基中引起的形态变化（从酵母到菌丝形成）。总之，这些结果表明，Ca-ap1 的给药对 CSC 诱导的生物膜形成具有显著的保护作用。
