Grigoryan Rosa, Costas-Rodríguez Marta, Van Wonterghem Elien, Vandenbroucke Roosmarijn E, Vanhaecke Frank
Atomic & Mass Spectrometry - A&MS Research Unit, Department of Chemistry, Ghent University, Ghent, Belgium.
Vlaams Instituut voor Biotechnologie (VIB) Center for Inflammation Research, Ghent, Belgium.
Front Med (Lausanne). 2021 Jul 23;8:664666. doi: 10.3389/fmed.2021.664666. eCollection 2021.
Endotoxemia induced in mice by intraperitoneal injection of lipopolysaccharide (LPS) leads to (neuro)inflammation and sepsis. Also the homeostasis of mineral elements can be altered through mechanisms that still are poorly understood. The isotopic composition of Mg and the concentrations of the minor elements Ca, K, Mg, Na, P, and S were determined in biological fluids and tissues of young (14-28 weeks) and aged (40-65 weeks) LPS-injected mice and age-matched controls to reveal potential effects of the LPS-induced infection. Blood plasma of young and aged LPS-injected mice showed a heavy Mg isotopic composition, as well as elevated Mg and P concentrations, compared to matched controls. The plasma Mg isotopic composition was correlated with the P concentration in aged mice. Also the liver Mg isotopic composition was strongly affected in the young and aged LPS-injected mice, while for aged mice, an additional effect on the urine Mg isotopic composition was established. These observations were hypothetically associated with liver inflammation and/or hepatotoxicity, and reduced urinary Mg excretion, respectively. Also a regional endotoxin-induced difference was observed in the brain Mg isotopic composition for the aged mice only, and was attributed to potential disruption of the blood-brain barrier.
通过腹腔注射脂多糖（LPS）在小鼠中诱导的内毒素血症会导致（神经）炎症和败血症。此外，矿物元素的稳态也可能通过仍知之甚少的机制发生改变。测定了年轻（14 - 28周）和老年（40 - 65周）LPS注射小鼠以及年龄匹配的对照小鼠的生物体液和组织中Mg的同位素组成以及微量元素Ca、K、Mg、Na、P和S的浓度，以揭示LPS诱导感染的潜在影响。与匹配的对照相比，年轻和老年LPS注射小鼠的血浆显示出较重的Mg同位素组成，以及升高的Mg和P浓度。老年小鼠血浆Mg同位素组成与P浓度相关。年轻和老年LPS注射小鼠的肝脏Mg同位素组成也受到强烈影响，而对于老年小鼠，尿液Mg同位素组成也受到额外影响。这些观察结果分别假设与肝脏炎症和/或肝毒性以及尿液Mg排泄减少有关。仅在老年小鼠的大脑Mg同位素组成中观察到区域内毒素诱导的差异，这归因于血脑屏障的潜在破坏。
