Chichila T M, Walters S M
Food and Drug Administration, Pesticides and Industrial Chemicals Research Center, Detroit, MI 48207.
J Assoc Off Anal Chem. 1991 Nov-Dec;74(6):961-7.
A method was developed for the determination of paraquat (PQ) and diquat (DQ) in high moisture food crops. Samples were digested with 6M HCl, and the herbicides were isolated from the digest using pH-controlled silica solid phase extraction. The analytes were then determined by ion-pairing liquid chromatography with a silica analytical column, sodium chloride as the ion-pairing reagent, and acetonitrile as the organic modifier. A diode array UV absorbance detector was used to simultaneously quantify PQ and DQ at their respective maximum absorbance wavelengths, 257 and 310 nm. Average recoveries of PQ and DQ standards from 4 different crops fortified at 0.01-0.50 ppm levels ranged from 79.3 to 104.8%.
开发了一种用于测定高水分粮食作物中百草枯（PQ）和敌草快（DQ）的方法。样品用6M盐酸消化，然后使用pH控制的硅胶固相萃取从消化液中分离出除草剂。然后通过离子对液相色谱法，以硅胶分析柱、氯化钠作为离子对试剂、乙腈作为有机改性剂来测定分析物。使用二极管阵列紫外吸光检测器在其各自的最大吸收波长257和310nm处同时定量PQ和DQ。在0.01 - 0.50ppm水平下对4种不同作物进行加标，PQ和DQ标准品的平均回收率为79.3%至104.8%。
