Takagi T, Rao P F, Kubota H
Institute for Protein Research, Osaka University.
J Biochem. 1987 Oct;102(4):681-4. doi: 10.1093/oxfordjournals.jbchem.a122105.
A modernized schlieren optics was applied to follow protein bands visually during polyacrylamide gel electrophoresis. A band containing as little as 0.3 microgram of a protein could be detected. Besides the protein bands, usually overlooked phenomena such as boundary migration of the buffer components could be visualized. As an example, electrophoretic patterns thus obtained for SDS-polyacrylamide gel electrophoresis with a discontinuous buffer system are presented. The use of a split-type colored filter was found to be useful for coloring a particular location on the gel red or blue depending on the sign of the refractive index gradient. This means of detection also seems useful in that, firstly, it makes electrophoretic mobility measurement more quantitative and, secondly, it allows localization of a protein band and sampling in the intact state.
一种现代化的纹影光学系统被应用于在聚丙烯酰胺凝胶电泳过程中直观地跟踪蛋白质条带。可以检测到含有低至0.3微克蛋白质的条带。除了蛋白质条带外，通常被忽视的现象，如缓冲液成分的边界迁移也可以可视化。作为一个例子，展示了使用不连续缓冲系统进行SDS-聚丙烯酰胺凝胶电泳时由此获得的电泳图谱。发现使用分裂型彩色滤光片有助于根据折射率梯度的符号将凝胶上的特定位置染成红色或蓝色。这种检测方法似乎也很有用，首先，它使电泳迁移率测量更具定量性，其次，它允许在完整状态下对蛋白质条带进行定位和取样。
